BIOPHYSICAL CHARACTERIZATION OF ASF/SF2’S INTERACTION WITH SPLICE SITE A7 IN THE HIV GENOME
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| Language: | English |
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Miami University / OhioLINK
2012
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=miami1354600074 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-miami13546000742021-08-03T05:41:42Z BIOPHYSICAL CHARACTERIZATION OF ASF/SF2’S INTERACTION WITH SPLICE SITE A7 IN THE HIV GENOME Kochert, Brent Andrew Biochemistry Biophysics Chemistry ASF SF2 Alternative Splicing Factor Splicing Factor 2 Exonic Splicing Enhancer 3 ESE3 Alternative Splicing Splice Site A7 Biophysical HIV Genome The HIV genome is synthesized as a 9.5kb polycistronic transcript that undergoes alternative splicing to produce the complete viral protein compliment. Within the HIV genome both donor and acceptor sites are coupled together to determine which of the over 40 transcripts are made and thus which viral proteins are produced. Host proteins are responsible for both the up regulation and down regulation of these sites and play a key role in viral protein production. ASF, is a serine arginine rich protein that is capable of binding splice site A7 and upregulating splicing. The RNA recognition motif domains (RRMs) were studied both separately and together with the complete splice site as well as exonic splicing enhancer 3 (ESE3). A major goal of this study was to identify which domain has a greater responsibility in binding the splice site. In order to understand this, a series of electrophoretic mobility shift assays were run. It is seen that RRM-1 has a higher affinity for both the complete splice site as well as ESE3. 2012-12-07 English text Miami University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=miami1354600074 http://rave.ohiolink.edu/etdc/view?acc_num=miami1354600074 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Biochemistry Biophysics Chemistry ASF SF2 Alternative Splicing Factor Splicing Factor 2 Exonic Splicing Enhancer 3 ESE3 Alternative Splicing Splice Site A7 Biophysical HIV Genome |
| spellingShingle |
Biochemistry Biophysics Chemistry ASF SF2 Alternative Splicing Factor Splicing Factor 2 Exonic Splicing Enhancer 3 ESE3 Alternative Splicing Splice Site A7 Biophysical HIV Genome Kochert, Brent Andrew BIOPHYSICAL CHARACTERIZATION OF ASF/SF2’S INTERACTION WITH SPLICE SITE A7 IN THE HIV GENOME |
| author |
Kochert, Brent Andrew |
| author_facet |
Kochert, Brent Andrew |
| author_sort |
Kochert, Brent Andrew |
| title |
BIOPHYSICAL CHARACTERIZATION OF ASF/SF2’S INTERACTION WITH SPLICE SITE A7 IN THE HIV GENOME |
| title_short |
BIOPHYSICAL CHARACTERIZATION OF ASF/SF2’S INTERACTION WITH SPLICE SITE A7 IN THE HIV GENOME |
| title_full |
BIOPHYSICAL CHARACTERIZATION OF ASF/SF2’S INTERACTION WITH SPLICE SITE A7 IN THE HIV GENOME |
| title_fullStr |
BIOPHYSICAL CHARACTERIZATION OF ASF/SF2’S INTERACTION WITH SPLICE SITE A7 IN THE HIV GENOME |
| title_full_unstemmed |
BIOPHYSICAL CHARACTERIZATION OF ASF/SF2’S INTERACTION WITH SPLICE SITE A7 IN THE HIV GENOME |
| title_sort |
biophysical characterization of asf/sf2’s interaction with splice site a7 in the hiv genome |
| publisher |
Miami University / OhioLINK |
| publishDate |
2012 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=miami1354600074 |
| work_keys_str_mv |
AT kochertbrentandrew biophysicalcharacterizationofasfsf2sinteractionwithsplicesitea7inthehivgenome |
| _version_ |
1719423742827823104 |