A Hydrostatic Pressure Perfusion System for Biological Systems

Bibliographic Details
Main Author:	Huff, Alison
Language:	English
Published:	Miami University / OhioLINK 2012
Subjects:	Biophysics Physics hydrostatic pressure perfusion system
Online Access:	http://rave.ohiolink.edu/etdc/view?acc_num=miami1343970397

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spelling	ndltd-OhioLink-oai-etd.ohiolink.edu-miami13439703972021-08-03T05:41:42Z A Hydrostatic Pressure Perfusion System for Biological Systems Huff, Alison Biophysics Physics hydrostatic pressure perfusion system A hydrostatic pressure perfusion system with two closed fluid reservoirs has been constructed and characterized. The system utilizes two stainless steel pressure generators connected to gearheads. Stepper motors are connected to the gearheads and are used to provide constant fluid flow throughout the system. We communicate with both motors through a hub, such that we can monitor the internal pressure of the system with a pressure gauge. Fluorescence has been used to detect changes in emission spectra between the different fluids in the reservoirs. We have also demonstrated that we can trap Saccharomyces cerevisiae, or baker's yeast, in a quartz capillary tube and detect spectral changes while monitoring the internal pressure of the system. 2012-08-09 English text Miami University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=miami1343970397 http://rave.ohiolink.edu/etdc/view?acc_num=miami1343970397 unrestricted This thesis or dissertation is protected by copyright: some rights reserved. It is licensed for use under a Creative Commons license. Specific terms and permissions are available from this document's record in the OhioLINK ETD Center.
collection	NDLTD
language	English
sources	NDLTD
topic	Biophysics Physics hydrostatic pressure perfusion system
spellingShingle	Biophysics Physics hydrostatic pressure perfusion system Huff, Alison A Hydrostatic Pressure Perfusion System for Biological Systems
author	Huff, Alison
author_facet	Huff, Alison
author_sort	Huff, Alison
title	A Hydrostatic Pressure Perfusion System for Biological Systems
title_short	A Hydrostatic Pressure Perfusion System for Biological Systems
title_full	A Hydrostatic Pressure Perfusion System for Biological Systems
title_fullStr	A Hydrostatic Pressure Perfusion System for Biological Systems
title_full_unstemmed	A Hydrostatic Pressure Perfusion System for Biological Systems
title_sort	hydrostatic pressure perfusion system for biological systems
publisher	Miami University / OhioLINK
publishDate	2012
url	http://rave.ohiolink.edu/etdc/view?acc_num=miami1343970397
work_keys_str_mv	AT huffalison ahydrostaticpressureperfusionsystemforbiologicalsystems AT huffalison hydrostaticpressureperfusionsystemforbiologicalsystems
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A Hydrostatic Pressure Perfusion System for Biological Systems

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