Polyphenols: Interactions with proteins and analytical methods

Polyphenols: Interactions with proteins and analytical methods

Bibliographic Details
Main Author: Trombley, John D.
Language:English
Published: Miami University / OhioLINK 2011
Subjects:
Analytical Chemistry
Biochemistry
Food Science
polyphenol
tannin
polyphenol-protein complex
antioxidant
Prussian blue
ab initio
epigallocatechin gallate
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=miami1322841396
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-miami13228413962021-08-03T05:41:25Z Polyphenols: Interactions with proteins and analytical methods Trombley, John D. Analytical Chemistry Biochemistry Food Science polyphenol tannin polyphenol-protein complex antioxidant Prussian blue ab initio epigallocatechin gallate Polyphenols are a diverse class of plant secondary metabolites with diverse chemical reactivities. The biological significance of polyphenol activities as antioxidants or protein-binding agents has not been clearly established. This dissertation describes the characterization of a model polyphenol-protein complex formed between (-) – epigallocatechin gallate (EGCg) and bovine serum albumin (BSA). In order to characterize the complex capillary electrophoresis (CE) methods were developed. An ion exchange chromatography method to purify the complex was developed. The complex appeared to comprise about 10 EGCg per BSA, covalently linked at cysteine, lysine and/or histidine residues. The dissertation also describes mathematical models that utilize ab initio calculated properties of polyphenols to predict the response in one of five common biochemical assays: BCA (bicinchoninic acid), MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide), ABTS (2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid), FRAP (ferric reducing antioxidant power) and the Prussian blue assay. 2011-12-05 English text Miami University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=miami1322841396 http://rave.ohiolink.edu/etdc/view?acc_num=miami1322841396 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Analytical Chemistry
Biochemistry
Food Science
polyphenol
tannin
polyphenol-protein complex
antioxidant
Prussian blue
ab initio
epigallocatechin gallate
spellingShingle Analytical Chemistry
Biochemistry
Food Science
polyphenol
tannin
polyphenol-protein complex
antioxidant
Prussian blue
ab initio
epigallocatechin gallate
Trombley, John D.
Polyphenols: Interactions with proteins and analytical methods
author Trombley, John D.
author_facet Trombley, John D.
author_sort Trombley, John D.
title Polyphenols: Interactions with proteins and analytical methods
title_short Polyphenols: Interactions with proteins and analytical methods
title_full Polyphenols: Interactions with proteins and analytical methods
title_fullStr Polyphenols: Interactions with proteins and analytical methods
title_full_unstemmed Polyphenols: Interactions with proteins and analytical methods
title_sort polyphenols: interactions with proteins and analytical methods
publisher Miami University / OhioLINK
publishDate 2011
url http://rave.ohiolink.edu/etdc/view?acc_num=miami1322841396
work_keys_str_mv AT trombleyjohnd polyphenolsinteractionswithproteinsandanalyticalmethods
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