The Effects of High Hydrostatic Pressures on NADH Conformation

The Effects of High Hydrostatic Pressures on NADH Conformation

Bibliographic Details
Main Author: Alquist, Erik James
Language:English
Published: Miami University / OhioLINK 2010
Subjects:
Biophysics
Physics
NADH
High Pressure
Conformation
Fluorescence
Volume change
Solvent denaturation
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=miami1281640692
id ndltd-OhioLink-oai-etd.ohiolink.edu-miami1281640692
record_format oai_dc
spelling ndltd-OhioLink-oai-etd.ohiolink.edu-miami12816406922021-08-03T05:41:09Z The Effects of High Hydrostatic Pressures on NADH Conformation Alquist, Erik James Biophysics Physics NADH High Pressure Conformation Fluorescence Volume change Solvent denaturation Reduced nicotinamide adenine dinucleotide (NADH) plays a central role in cellularmetabolism via a redox reaction. Its conformation is physiologically significant asNADH takes a folded conformation when free and an unfolded conformation whenprotein bound. This study uses fluorescent emission spectroscopy and solventdenaturation to examine pressure’s role in the equilibrium conformation of NADH insolution (10 and 20 μM in MOPS buffer, pH 7.4). Quartz capillary-based high-pressurechambers house samples up to 51 MPa which are excited with a 337 nm nitrogen laser.Using a two-state model, the free energy of unfolding is determined and the volumechange of unfolding is measured to be 24.9 ml/mol assuming an Arrhenius relationship.The validities of the two-state model and the selected concentration range are assessed.Results are significant to understanding pressure effects on cellular metabolism and forfuture studies probing the fluorescent signals of cells under pressure. 2010-08-17 English text Miami University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=miami1281640692 http://rave.ohiolink.edu/etdc/view?acc_num=miami1281640692 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Biophysics
Physics
NADH
High Pressure
Conformation
Fluorescence
Volume change
Solvent denaturation
spellingShingle Biophysics
Physics
NADH
High Pressure
Conformation
Fluorescence
Volume change
Solvent denaturation
Alquist, Erik James
The Effects of High Hydrostatic Pressures on NADH Conformation
author Alquist, Erik James
author_facet Alquist, Erik James
author_sort Alquist, Erik James
title The Effects of High Hydrostatic Pressures on NADH Conformation
title_short The Effects of High Hydrostatic Pressures on NADH Conformation
title_full The Effects of High Hydrostatic Pressures on NADH Conformation
title_fullStr The Effects of High Hydrostatic Pressures on NADH Conformation
title_full_unstemmed The Effects of High Hydrostatic Pressures on NADH Conformation
title_sort effects of high hydrostatic pressures on nadh conformation
publisher Miami University / OhioLINK
publishDate 2010
url http://rave.ohiolink.edu/etdc/view?acc_num=miami1281640692
work_keys_str_mv AT alquisterikjames theeffectsofhighhydrostaticpressuresonnadhconformation
AT alquisterikjames effectsofhighhydrostaticpressuresonnadhconformation
_version_ 1719423573115797504

Similar Items