METHODS FOR IN SITU PIEZOPHYSIOLOGICAL STUDIES: OPTICAL SECTIONING VIA STRUCTURED ILLUMINATION AND FLUORESCENCE BASED CHARACTERIZATION OF NADH CONFORMATION

METHODS FOR IN SITU PIEZOPHYSIOLOGICAL STUDIES: OPTICAL SECTIONING VIA STRUCTURED ILLUMINATION AND FLUORESCENCE BASED CHARACTERIZATION OF NADH CONFORMATION

Bibliographic Details
Main Author: Farooqi, Mohammed Junaid
Language:English
Published: Miami University / OhioLINK 2009
Subjects:
Biophysics
Optics
Physics
Optical Sectioning
Structured Illumination
NADH
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=miami1249225952
id ndltd-OhioLink-oai-etd.ohiolink.edu-miami1249225952
record_format oai_dc
spelling ndltd-OhioLink-oai-etd.ohiolink.edu-miami12492259522021-08-03T05:40:48Z METHODS FOR IN SITU PIEZOPHYSIOLOGICAL STUDIES: OPTICAL SECTIONING VIA STRUCTURED ILLUMINATION AND FLUORESCENCE BASED CHARACTERIZATION OF NADH CONFORMATION Farooqi, Mohammed Junaid Biophysics Optics Physics Optical Sectioning Structured Illumination NADH In this thesis thermodynamic properties of NADH (dihydronicotinamide adenine dinucleotide) conformational states at 1 atm pressure were characterized in a pH buffer MOPS (pH = 7.5) with varying concentrations of denaturant (methanol) concentrations. Linear model and spectral decomposition model were developed to calculate fraction-unfolded values of NADH in different methanol concentrations. An optical sectioning system using a structured illumination setup based on previous literature was built for use with a wide-field microscope. The sectioning ability of the system was shown in slide and capillary mounted paper fibers. The illumination setup was also used to a acquire sectioned images by varying the z-axis coordinates and building a 3D image of the slide mounted paper fibers, and used to show a comparison between the conventional and sectioned 3D image. 2009-08-02 English text Miami University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=miami1249225952 http://rave.ohiolink.edu/etdc/view?acc_num=miami1249225952 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Biophysics
Optics
Physics
Optical Sectioning
Structured Illumination
NADH
spellingShingle Biophysics
Optics
Physics
Optical Sectioning
Structured Illumination
NADH
Farooqi, Mohammed Junaid
METHODS FOR IN SITU PIEZOPHYSIOLOGICAL STUDIES: OPTICAL SECTIONING VIA STRUCTURED ILLUMINATION AND FLUORESCENCE BASED CHARACTERIZATION OF NADH CONFORMATION
author Farooqi, Mohammed Junaid
author_facet Farooqi, Mohammed Junaid
author_sort Farooqi, Mohammed Junaid
title METHODS FOR IN SITU PIEZOPHYSIOLOGICAL STUDIES: OPTICAL SECTIONING VIA STRUCTURED ILLUMINATION AND FLUORESCENCE BASED CHARACTERIZATION OF NADH CONFORMATION
title_short METHODS FOR IN SITU PIEZOPHYSIOLOGICAL STUDIES: OPTICAL SECTIONING VIA STRUCTURED ILLUMINATION AND FLUORESCENCE BASED CHARACTERIZATION OF NADH CONFORMATION
title_full METHODS FOR IN SITU PIEZOPHYSIOLOGICAL STUDIES: OPTICAL SECTIONING VIA STRUCTURED ILLUMINATION AND FLUORESCENCE BASED CHARACTERIZATION OF NADH CONFORMATION
title_fullStr METHODS FOR IN SITU PIEZOPHYSIOLOGICAL STUDIES: OPTICAL SECTIONING VIA STRUCTURED ILLUMINATION AND FLUORESCENCE BASED CHARACTERIZATION OF NADH CONFORMATION
title_full_unstemmed METHODS FOR IN SITU PIEZOPHYSIOLOGICAL STUDIES: OPTICAL SECTIONING VIA STRUCTURED ILLUMINATION AND FLUORESCENCE BASED CHARACTERIZATION OF NADH CONFORMATION
title_sort methods for in situ piezophysiological studies: optical sectioning via structured illumination and fluorescence based characterization of nadh conformation
publisher Miami University / OhioLINK
publishDate 2009
url http://rave.ohiolink.edu/etdc/view?acc_num=miami1249225952
work_keys_str_mv AT farooqimohammedjunaid methodsforinsitupiezophysiologicalstudiesopticalsectioningviastructuredilluminationandfluorescencebasedcharacterizationofnadhconformation
_version_ 1719423467320770560

Similar Items