Studies on Cold Resistance in Palms: Analysis of <i>CBF</i>-Like Genes

Studies on Cold Resistance in Palms: Analysis of <i>CBF</i>-Like Genes

Bibliographic Details
Main Author: Lu, Li
Language:English
Published: Miami University / OhioLINK 2006
Subjects:
CBF/DREB1
cold acclimation
cold resistance
palm
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=miami1165601341
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-miami11656013412021-08-03T05:39:59Z Studies on Cold Resistance in Palms: Analysis of <i>CBF</i>-Like Genes Lu, Li CBF/DREB1 cold acclimation cold resistance palm Cold resistance of plants is an important characteristic that restricts plant distribution, growth and biomass productivity. Cold acclimation is a phenomenon that occurs in some plants as an increase in cold tolerance upon exposure to low, non-freezing temperatures. The <i>CBF/DREB1</i> gene family is a small group of transcription factors that play key roles in cold acclimation. The <i>CBF/DREB1</i> genes were first identified from the model plant <i>Arabidopsis</i>, then from many economically important plant species including grape, tomato, corn, rice, and barley. Overexpression of <i>CBF/DREB1</i> genes induces multiple components of cold acclimation and thus increases plant cold tolerance even without cold stimulus. To systematically study the cold resistance mechanism of palms, <i>CBF/DREB1</i> orthologs were isolated from various palms, including both cold-resistant and cold-sensitive species. This is the first report of <i>CBF/DREB1</i> genes in the Palmae family. Analyses of DNA and putative protein sequences confirmed that the palm CBF/DREB1 has similar structures with known CBF proteins. To further investigate the functions of the palm <i>CBF/DREB1</i> genes, the expression patterns of two Rhapidophyllum <i>hystrix CBF</i> orthologs, <i>RhCBF1</i> and <i>RhCBF2</i>, were analyzed with reverse transcription and real-time PCR. The expressions of the two <i>RhCBFs</i> were constitutive yet cold-inducible, which provided further evidence of the involvement of these genes in cold acclimation response of palms. The two <i>RhCBF</i> genes were transformed into Arabidopsis. The transgenic <i>Arabidopsis</i> plants showed increased cold tolerance, typical growth retardation of <i>CBF</i>-overexpessing plants, and other multiple <i>CBF</i>-related cellular changes. The results indicated that <i>RhCBFs</i> had functional similarity with <i>Arabidopsis CBF</i> genes also. 2006-12-08 English text Miami University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=miami1165601341 http://rave.ohiolink.edu/etdc/view?acc_num=miami1165601341 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic CBF/DREB1
cold acclimation
cold resistance
palm
spellingShingle CBF/DREB1
cold acclimation
cold resistance
palm
Lu, Li
Studies on Cold Resistance in Palms: Analysis of <i>CBF</i>-Like Genes
author Lu, Li
author_facet Lu, Li
author_sort Lu, Li
title Studies on Cold Resistance in Palms: Analysis of <i>CBF</i>-Like Genes
title_short Studies on Cold Resistance in Palms: Analysis of <i>CBF</i>-Like Genes
title_full Studies on Cold Resistance in Palms: Analysis of <i>CBF</i>-Like Genes
title_fullStr Studies on Cold Resistance in Palms: Analysis of <i>CBF</i>-Like Genes
title_full_unstemmed Studies on Cold Resistance in Palms: Analysis of <i>CBF</i>-Like Genes
title_sort studies on cold resistance in palms: analysis of <i>cbf</i>-like genes
publisher Miami University / OhioLINK
publishDate 2006
url http://rave.ohiolink.edu/etdc/view?acc_num=miami1165601341
work_keys_str_mv AT luli studiesoncoldresistanceinpalmsanalysisoficbfilikegenes
_version_ 1719423282328895488

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