Role of Cofilin, an Actin Cytoskeletal Protein, in Ischemic Conditions: Potential Therapeutic Target for Ischemic Stroke

Role of Cofilin, an Actin Cytoskeletal Protein, in Ischemic Conditions: Potential Therapeutic Target for Ischemic Stroke

Bibliographic Details
Main Author: Madineni, Anusha
Language:English
Published: University of Toledo Health Science Campus / OhioLINK 2013
Subjects:
Cellular Biology
Neurosciences
Pharmacology
Pharmaceuticals
Cofilin
Neurodegeneration
Cerebral ischemia
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=mco1364331841
id ndltd-OhioLink-oai-etd.ohiolink.edu-mco1364331841
record_format oai_dc
spelling ndltd-OhioLink-oai-etd.ohiolink.edu-mco13643318412021-08-03T05:21:10Z Role of Cofilin, an Actin Cytoskeletal Protein, in Ischemic Conditions: Potential Therapeutic Target for Ischemic Stroke Madineni, Anusha Cellular Biology Neurosciences Pharmacology Pharmaceuticals Cofilin Neurodegeneration Cerebral ischemia Cerebral ischemia or stroke is a condition associated with decreased blood supply to brain leading to death of neurons. It is associated with a diverse cascade of responses involving both degenerative and regenerative mechanisms. Actin cytoskeleton changes play a key role in mediating these responses. Cofilin, an actin cytoskeletal severing protein is known to be involved in early stages of apoptotic cell death. Evidence supports its intervention in progression of disease states like Alzheimer’s and ischemic kidney disease. We have hypothesized possible involvement of cofilin in cerebral ischemia and neurodegeneration. To investigate the potential actin cytoskeletal changes induced by cofilin in ischemia, various ischemic models such as oxidative stress, oxygen glucose deprivation (OGD) in vitro and permanent middle cerebral artery occlusion (pMCAO) in vivo were used. The expression profile studies by immunoblotting and fluorescence methods displayed decrease in phosphocofilin (inactive form of cofilin) levels with all models of ischemia implying stress induced cofilin activation. Furthermore, calcineurin and slingshot 1L (SSH) phosphatases were found to be the signaling mediators of the cofilin activation pathway in ischemia elucidated by our inhibitor studies. These results strongly support cofilin role in cerebral ischemia induced actin cytoskeleton alterations leading to cell damage. We believe targeting this protein mediator has a potential for therapeutic intervention in stroke. 2013-05-20 English text University of Toledo Health Science Campus / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=mco1364331841 http://rave.ohiolink.edu/etdc/view?acc_num=mco1364331841 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Cellular Biology
Neurosciences
Pharmacology
Pharmaceuticals
Cofilin
Neurodegeneration
Cerebral ischemia
spellingShingle Cellular Biology
Neurosciences
Pharmacology
Pharmaceuticals
Cofilin
Neurodegeneration
Cerebral ischemia
Madineni, Anusha
Role of Cofilin, an Actin Cytoskeletal Protein, in Ischemic Conditions: Potential Therapeutic Target for Ischemic Stroke
author Madineni, Anusha
author_facet Madineni, Anusha
author_sort Madineni, Anusha
title Role of Cofilin, an Actin Cytoskeletal Protein, in Ischemic Conditions: Potential Therapeutic Target for Ischemic Stroke
title_short Role of Cofilin, an Actin Cytoskeletal Protein, in Ischemic Conditions: Potential Therapeutic Target for Ischemic Stroke
title_full Role of Cofilin, an Actin Cytoskeletal Protein, in Ischemic Conditions: Potential Therapeutic Target for Ischemic Stroke
title_fullStr Role of Cofilin, an Actin Cytoskeletal Protein, in Ischemic Conditions: Potential Therapeutic Target for Ischemic Stroke
title_full_unstemmed Role of Cofilin, an Actin Cytoskeletal Protein, in Ischemic Conditions: Potential Therapeutic Target for Ischemic Stroke
title_sort role of cofilin, an actin cytoskeletal protein, in ischemic conditions: potential therapeutic target for ischemic stroke
publisher University of Toledo Health Science Campus / OhioLINK
publishDate 2013
url http://rave.ohiolink.edu/etdc/view?acc_num=mco1364331841
work_keys_str_mv AT madinenianusha roleofcofilinanactincytoskeletalproteininischemicconditionspotentialtherapeutictargetforischemicstroke
_version_ 1719418643166527488

Similar Items