Development, Characterization and Evaluation of Solid Lipid Nanoparticles as a Potential Anticancer Drug Delivery System

Bibliographic Details
Main Author: Patel, Meghavi
Language:English
Published: University of Toledo Health Science Campus / OhioLINK 2012
Subjects:
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=mco1352944517
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-mco13529445172021-08-03T05:38:54Z Development, Characterization and Evaluation of Solid Lipid Nanoparticles as a Potential Anticancer Drug Delivery System Patel, Meghavi Pharmaceuticals Pharmacy Sciences Solid lipid nanoparticles 5-fluorouracil Anticancer Glyceryl monostearate Dynamic light scattering Transmission electron microscopy Differential scanning calorimetry Solid lipid nanoparticles (SLNs) consist of spherical solid lipid particles in the nanometer size range, which are dispersed in water or in an aqueous surfactant solution. SLN technology represents a promising new approach to deliver hydrophilic as well as lipophilic drugs. The commercialization of SLN technology remains limited despite numerous efforts from researchers. The purpose of this research was to advance SLN preparation methodology by investigating the feasibility of preparing glyceryl monostearate (GMS) nanoparticles by using three preparation methods namely microemulsion technique, magnetic stirring technique and temperature modulated solidification technique of which the latter two were developed in our laboratory. An anticancer drug 5-fluorouracil was incorporated in the SLNs prepared via the temperature modulated solidification process. Optimization of the magnetic stirring process was performed to evaluate how the physicochemical properties of the SLN was influenced by systematically varying process parameters including concentration of the lipid, concentration of the surfactant, type of surfactant, time of stirring and temperature of storage. The results demonstrated 1:2 GMS to tween 80 ratio, 150 ml dispersion medium and 45 min stirring at 4000 RPM speed provided an optimum formulation via the temperature modulated solidification process. SLN dispersions were lyophilized to stabilize the solid lipid nanoparticles and the lyophilizates exhibited good redispersibility. The SLNs were characterized by particle size analysis via dynamic light scattering (DLS), zeta potential, transmission electron microscopy (TEM), differential scanning calorimetry (DSC), drug encapsulation efficiency and in vitro drug release studies. Particle size of SLN dispersion prepared via the three preparation techniques was approximately 66 nm and that of redispersed lyophilizates was below 500 nm. TEM images showed spherical to oval particles that were less dense in the core with a well-defined shell and the particle size was in agreement with the particle size analysis data obtained by DLS. DSC thermograms of the lyophilized SLNs indicate a reduction in the crystallinity order of GMS particles. The drug encapsulation efficiency was found to be approximately 30%. In vitro drug release studies from redispersed lyophilized SLNs showed that 17 % of the encapsulated drug was released within 2 h. The SLNs prepared in our lab demonstrated characteristics that can potentially be utilized in an anticancer drug delivery system. Future in vitro cell culture and in vivo animal model studies will delineate compatibility and utility of these formulations in biological systems. 2012 English text University of Toledo Health Science Campus / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=mco1352944517 http://rave.ohiolink.edu/etdc/view?acc_num=mco1352944517 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Pharmaceuticals
Pharmacy Sciences
Solid lipid nanoparticles
5-fluorouracil
Anticancer
Glyceryl monostearate
Dynamic light scattering
Transmission electron microscopy
Differential scanning calorimetry
spellingShingle Pharmaceuticals
Pharmacy Sciences
Solid lipid nanoparticles
5-fluorouracil
Anticancer
Glyceryl monostearate
Dynamic light scattering
Transmission electron microscopy
Differential scanning calorimetry
Patel, Meghavi
Development, Characterization and Evaluation of Solid Lipid Nanoparticles as a Potential Anticancer Drug Delivery System
author Patel, Meghavi
author_facet Patel, Meghavi
author_sort Patel, Meghavi
title Development, Characterization and Evaluation of Solid Lipid Nanoparticles as a Potential Anticancer Drug Delivery System
title_short Development, Characterization and Evaluation of Solid Lipid Nanoparticles as a Potential Anticancer Drug Delivery System
title_full Development, Characterization and Evaluation of Solid Lipid Nanoparticles as a Potential Anticancer Drug Delivery System
title_fullStr Development, Characterization and Evaluation of Solid Lipid Nanoparticles as a Potential Anticancer Drug Delivery System
title_full_unstemmed Development, Characterization and Evaluation of Solid Lipid Nanoparticles as a Potential Anticancer Drug Delivery System
title_sort development, characterization and evaluation of solid lipid nanoparticles as a potential anticancer drug delivery system
publisher University of Toledo Health Science Campus / OhioLINK
publishDate 2012
url http://rave.ohiolink.edu/etdc/view?acc_num=mco1352944517
work_keys_str_mv AT patelmeghavi developmentcharacterizationandevaluationofsolidlipidnanoparticlesasapotentialanticancerdrugdeliverysystem
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