The Effect of Glucose on Beta-Catenin Expression in Diabetic and Normal Cells

The Effect of Glucose on Beta-Catenin Expression in Diabetic and Normal Cells

Bibliographic Details
Main Author: Stahl, Rachel
Language:English
Published: Marietta College Honors Theses / OhioLINK 2017
Subjects:
Biochemistry
Beta-catenin pathway
diabetic cells
cancerous cells
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=marhonors1496139881597792
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-marhonors14961398815977922021-08-03T07:02:43Z The Effect of Glucose on Beta-Catenin Expression in Diabetic and Normal Cells Stahl, Rachel Biochemistry Beta-catenin pathway diabetic cells cancerous cells The canonical Wnt/ß-catenin pathway is highly conserved throughout most species, and regulates cell growth, survival, and differentiation. Recently, abnormalities to this pathway have been associated with various cancers and in diabetes. One of the abnormalities is an increase in Wnt signaling, resulting in increased ß-catenin within the cell. Abnormal glucose levels, characteristic of diabetic patients, are proposed to trigger the up-regulation of the Wnt pathway. This study focuses on the effect of increased and decreased glucose levels on COS (healthy) and 3T3-L1 (diabetic) cells to determine if a correlation exists. Cells were treated with 12.5mM, 25mM, and 50mM glucose-treated media and then lysed for protein extraction. Western blot was utilized to determine differences in ß-catenin expression. ß-actin was used to standardize ß-catenin. COS cells treated with 12.5mM glucose expressed 43% ß-catenin compared to the 25mM glucose-treated cells. 3T3-L1 cells expressed 46% of total ß-catenin when treated with 12.5mM glucose, and 38% of total ß-catenin when treated with 50mM glucose. Future research using cancerous cells instead of diabetic cells will be able to determine if a connection between cancerous and diabetic cells through the Wnt/ß-catenin pathway exists. 2017-05-30 English text Marietta College Honors Theses / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=marhonors1496139881597792 http://rave.ohiolink.edu/etdc/view?acc_num=marhonors1496139881597792 unrestricted This thesis or dissertation is protected by copyright: some rights reserved. It is licensed for use under a Creative Commons license. Specific terms and permissions are available from this document's record in the OhioLINK ETD Center.
collection NDLTD
language English
sources NDLTD
topic Biochemistry
Beta-catenin pathway
diabetic cells
cancerous cells
spellingShingle Biochemistry
Beta-catenin pathway
diabetic cells
cancerous cells
Stahl, Rachel
The Effect of Glucose on Beta-Catenin Expression in Diabetic and Normal Cells
author Stahl, Rachel
author_facet Stahl, Rachel
author_sort Stahl, Rachel
title The Effect of Glucose on Beta-Catenin Expression in Diabetic and Normal Cells
title_short The Effect of Glucose on Beta-Catenin Expression in Diabetic and Normal Cells
title_full The Effect of Glucose on Beta-Catenin Expression in Diabetic and Normal Cells
title_fullStr The Effect of Glucose on Beta-Catenin Expression in Diabetic and Normal Cells
title_full_unstemmed The Effect of Glucose on Beta-Catenin Expression in Diabetic and Normal Cells
title_sort effect of glucose on beta-catenin expression in diabetic and normal cells
publisher Marietta College Honors Theses / OhioLINK
publishDate 2017
url http://rave.ohiolink.edu/etdc/view?acc_num=marhonors1496139881597792
work_keys_str_mv AT stahlrachel theeffectofglucoseonbetacateninexpressionindiabeticandnormalcells
AT stahlrachel effectofglucoseonbetacateninexpressionindiabeticandnormalcells
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