Extraction and Functional Assay of Cloned Thymopentin 5

Extraction and Functional Assay of Cloned Thymopentin 5

Bibliographic Details
Main Author: Greenstein, Rebecca S.
Language:English
Published: Marietta College Honors Theses / OhioLINK 2015
Subjects:
Biochemistry
Immunology
Genetics
Cellular Biology
genetic engineering
thymopentin 5
interleukin-2
pETFVA- vector
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=marhonors1429894251
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-marhonors14298942512021-08-03T06:30:45Z Extraction and Functional Assay of Cloned Thymopentin 5 Greenstein, Rebecca S. Biochemistry Immunology Genetics Cellular Biology genetic engineering thymopentin 5 interleukin-2 pETFVA- vector Thymopentin 5 is a synthetic immunomodulary peptide derived from the thymic hormone known as thymopoietin. It has been demonstrated that thymopentin 5 can be used to enhance immune response to vaccinations and cancers, rectify immune system imbalances, and increase the production of an important cytokine called interleukin-2. Organic peptide synthesis is the current method used to produce thymopentin 5, which is a time-consuming and expensive process that does not always result in a functional protein product. If thymopentin 5 can instead be produced using genetic engineering the peptide can be synthesized in a faster and less costly manner with a higher degree of folding accuracy. Previous work has been performed to insert the DNA for thymopentin 5 in to the pETFVA- vector. This research will attempt to transfect this vector in to COS-1 monkey kidney cells and determine whether a functional thymopentin 5 protein product is produced. In this project, the pETFVA- vector containing the thymopentin 5 DNA was transfected in to COS-1 cells; however, no thymopentin 5 protein was observed and there was no difference in interleukin-2 expression between transfected and non-transfected COS-1 cells. 2015-04-28 English text Marietta College Honors Theses / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=marhonors1429894251 http://rave.ohiolink.edu/etdc/view?acc_num=marhonors1429894251 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Biochemistry
Immunology
Genetics
Cellular Biology
genetic engineering
thymopentin 5
interleukin-2
pETFVA- vector
spellingShingle Biochemistry
Immunology
Genetics
Cellular Biology
genetic engineering
thymopentin 5
interleukin-2
pETFVA- vector
Greenstein, Rebecca S.
Extraction and Functional Assay of Cloned Thymopentin 5
author Greenstein, Rebecca S.
author_facet Greenstein, Rebecca S.
author_sort Greenstein, Rebecca S.
title Extraction and Functional Assay of Cloned Thymopentin 5
title_short Extraction and Functional Assay of Cloned Thymopentin 5
title_full Extraction and Functional Assay of Cloned Thymopentin 5
title_fullStr Extraction and Functional Assay of Cloned Thymopentin 5
title_full_unstemmed Extraction and Functional Assay of Cloned Thymopentin 5
title_sort extraction and functional assay of cloned thymopentin 5
publisher Marietta College Honors Theses / OhioLINK
publishDate 2015
url http://rave.ohiolink.edu/etdc/view?acc_num=marhonors1429894251
work_keys_str_mv AT greensteinrebeccas extractionandfunctionalassayofclonedthymopentin5
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