Development of Software and Hardware Tools to Improve Direct Mass-Spectrometric Analyses
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Kent State University / OhioLINK
2017
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=kent1493053646961652 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-kent14930536469616522021-08-03T07:02:08Z Development of Software and Hardware Tools to Improve Direct Mass-Spectrometric Analyses You, Yi Chemistry The emergence of direct-analysis mass spectrometry (MS) tool, including ambient desorption/ionization (ADI) methods, have enabled high-throughput sample screening and identification with minimal sample preparation. Although important physiochemical properties of the analytes are encoded in to the time-domain, time information is commonly discarded during the data interpretation of ADI-MS due to the large random variances. In this work, a mathematical approach, termed cross correlation, was used to gauge the similarities between the ion chronograms in sillico in near real-time, where analyte ions that were highly correlated were recognized and extracted, whereas the background ions were removed from the complicated ADI mass spectra. Moreover, the correlation-based ion recognition was expanded to tandem MS analyses. The fragmentation occurred in the vacuum region of a mass spectrometer; thus the fragment ions inherited the time-domain features of the analyte during the ADI process. In this work, tandem mass spectra of each analyte within a simultaneously introduced sample mixture were isolated/extracted. Furthermore, the abundance of reagent ions during ADI is directly related to the sensitivity and the limit-of-detection. In the present work, we demonstrate the development of ion manipulation/focusing at atmospheric pressure with ultrasound, obviating the need for high voltages and/or complicated ion-optic geometries. 2017-04-25 English text Kent State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=kent1493053646961652 http://rave.ohiolink.edu/etdc/view?acc_num=kent1493053646961652 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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NDLTD |
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English |
| sources |
NDLTD |
| topic |
Chemistry |
| spellingShingle |
Chemistry You, Yi Development of Software and Hardware Tools to Improve Direct Mass-Spectrometric Analyses |
| author |
You, Yi |
| author_facet |
You, Yi |
| author_sort |
You, Yi |
| title |
Development of Software and Hardware Tools to Improve Direct Mass-Spectrometric Analyses |
| title_short |
Development of Software and Hardware Tools to Improve Direct Mass-Spectrometric Analyses |
| title_full |
Development of Software and Hardware Tools to Improve Direct Mass-Spectrometric Analyses |
| title_fullStr |
Development of Software and Hardware Tools to Improve Direct Mass-Spectrometric Analyses |
| title_full_unstemmed |
Development of Software and Hardware Tools to Improve Direct Mass-Spectrometric Analyses |
| title_sort |
development of software and hardware tools to improve direct mass-spectrometric analyses |
| publisher |
Kent State University / OhioLINK |
| publishDate |
2017 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=kent1493053646961652 |
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AT youyi developmentofsoftwareandhardwaretoolstoimprovedirectmassspectrometricanalyses |
| _version_ |
1719452185417220096 |