Sex Differences of Neurotransporters Within the Nigrostriatal Dopaminergic System
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Kent State University / OhioLINK
2008
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=kent1227327111 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-kent12273271112021-08-03T05:36:49Z Sex Differences of Neurotransporters Within the Nigrostriatal Dopaminergic System Ji, Jing Biomedical Research DAT DA VMAT-2 mice DA output DOPAC Reserpine <p>The purpose was to examine the sex differences of VMAT-2 and DAT of the NSDA system in female and male mice. Two series experiments were performed: the first consisted of an in vivo experiment using reserpine-treated CD-1 mice; the second was in vitro experiment using DAT wild type and heterozygous mice. For the in vivo experiments, after treatment with reserpine, male mice showed significantly greater DA concentrations and K+-evoked DA output from the striatum. By contrast, no statistically significant sex differences were present in the response to MA-evoked DA output in reserpine-treated mice. As for the in vitro experiments, I measured DA and DOPAC contents in the corpus striatum and olfactory tubercle, and DA output induced by K+ and MA, and we compared effects of the VMAT-2 inhibiting drug reserpine upon the striatal dopaminergic function among male and female DAT wild type (+/+) and heterozygous (+/-), intact and gonadectomized mice. Striatal DA and DOPAC concentrations of female +/- DAT mice were significantly decreased as compared with wild type (+/+) controls and male +/- DAT mice. MA-evoked DA was increased in male and female +/- mice. While MA-evoked DA was significantly increased in +/+ males versus +/+ females, the +/- females showed the highest DA responses; thereby showing a reversal in the results seen in wild-type conditions. Potassium-stimulated DA was increased in male and female +/- DAT mice and maximally stimulated DA was obtained from +/- DAT females, although these mice showed the lowest DA concentrations. Infusion of reserpine-evoked DA output was significantly greater from intact +/+ and +/- female compared to male mice. The DA output profile differed markedly between +/+ and +/- females, but no such qualitative differences were present in males. Significantly increased reserpine-evoked DA responses were also obtained in +/+ and +/- gonadectomized female mice treated or not with estrogen as compared with respective responses from males. I can conclude that: 1) a deficiency in the DAT interacts with the sex of subject, 2) +/- DAT females show more extreme changes in dopaminergic responses, 3) females possess a more active VMAT-2/DA storage capacity, 4) the two transporter systems, DAT and VMAT-2, may be linked with each other and be affected differentially by the sex, 5) estrogen might contribute to this interaction between the DAT and VMAT-2, 6) the importance for understanding the sex differences present in the NSDA system in both healthy and diseased conditions.</p> 2008-11-24 English text Kent State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=kent1227327111 http://rave.ohiolink.edu/etdc/view?acc_num=kent1227327111 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
| collection |
NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Biomedical Research DAT DA VMAT-2 mice DA output DOPAC Reserpine |
| spellingShingle |
Biomedical Research DAT DA VMAT-2 mice DA output DOPAC Reserpine Ji, Jing Sex Differences of Neurotransporters Within the Nigrostriatal Dopaminergic System |
| author |
Ji, Jing |
| author_facet |
Ji, Jing |
| author_sort |
Ji, Jing |
| title |
Sex Differences of Neurotransporters Within the Nigrostriatal Dopaminergic System |
| title_short |
Sex Differences of Neurotransporters Within the Nigrostriatal Dopaminergic System |
| title_full |
Sex Differences of Neurotransporters Within the Nigrostriatal Dopaminergic System |
| title_fullStr |
Sex Differences of Neurotransporters Within the Nigrostriatal Dopaminergic System |
| title_full_unstemmed |
Sex Differences of Neurotransporters Within the Nigrostriatal Dopaminergic System |
| title_sort |
sex differences of neurotransporters within the nigrostriatal dopaminergic system |
| publisher |
Kent State University / OhioLINK |
| publishDate |
2008 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=kent1227327111 |
| work_keys_str_mv |
AT jijing sexdifferencesofneurotransporterswithinthenigrostriataldopaminergicsystem |
| _version_ |
1719422475736973312 |