Functional Consequences of Physical Interactions Between PriA and PriB in DNA Replication Restart Pathways in Neisseria Gonorrhoeae

Functional Consequences of Physical Interactions Between PriA and PriB in DNA Replication Restart Pathways in Neisseria Gonorrhoeae

Bibliographic Details
Main Author: Feng, Cui
Language:English
Published: University of Dayton / OhioLINK 2011
Subjects:
Biochemistry
DNA replication restart
Neisseria Gonorrhoeae
PriA
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=dayton1323795871
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-dayton13237958712021-08-03T05:35:56Z Functional Consequences of Physical Interactions Between PriA and PriB in DNA Replication Restart Pathways in Neisseria Gonorrhoeae Feng, Cui Biochemistry DNA replication restart Neisseria Gonorrhoeae PriA DNA replication restart pathways enable bacterial cells to reinitiate DNA replication when replication has been disrupted due to encounters with DNA damage, thereby allowing complete and faithful duplication of the cell's genetic information. Neisseria gonorrhoeae is a bacterium that is highly adapted to survive oxidative damage to its DNA incurred by attack from immune cells in infected individuals, suggesting that DNA replication restart pathways might play a critical role in N. gonorrhoeae pathogenicity. The bacterial helicase, PriA, is a key primosome protein that plays essential roles in DNA replication restart pathways. However, little is known of the mechanism by which PriA performs these roles in N. gonorrhoeae. I performed equilibrium DNA binding assays and DNA unwinding assays to provide insight into the mechanisms by which PriA functions in DNA replication restart pathways. I report that DNA binding by PriA is strongly dependent on the structure of the DNA. DNA substrates that resemble a DNA replication fork with a three-way branch are bound with higher affinity than partial duplex structures or single-stranded DNA. PriA-catalyzed DNA unwinding is also DNA structure-specific, and PriA-catalyzed unwinding decreases upon increasing the length of the duplex DNA, indicating that PriA is a low-processivity helicase. Another primosome protein, PriB, strongly stimulates the helicase activity of PriA, and this activity might facilitate reloading of the replication machinery by PriA at repaired replication forks. Stimulation of PriA by PriB appears to occur through a mechanism that is distinct from that used by the well-studied E. coli primosome proteins. 2011 English text University of Dayton / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=dayton1323795871 http://rave.ohiolink.edu/etdc/view?acc_num=dayton1323795871 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Biochemistry
DNA replication restart
Neisseria Gonorrhoeae
PriA
spellingShingle Biochemistry
DNA replication restart
Neisseria Gonorrhoeae
PriA
Feng, Cui
Functional Consequences of Physical Interactions Between PriA and PriB in DNA Replication Restart Pathways in Neisseria Gonorrhoeae
author Feng, Cui
author_facet Feng, Cui
author_sort Feng, Cui
title Functional Consequences of Physical Interactions Between PriA and PriB in DNA Replication Restart Pathways in Neisseria Gonorrhoeae
title_short Functional Consequences of Physical Interactions Between PriA and PriB in DNA Replication Restart Pathways in Neisseria Gonorrhoeae
title_full Functional Consequences of Physical Interactions Between PriA and PriB in DNA Replication Restart Pathways in Neisseria Gonorrhoeae
title_fullStr Functional Consequences of Physical Interactions Between PriA and PriB in DNA Replication Restart Pathways in Neisseria Gonorrhoeae
title_full_unstemmed Functional Consequences of Physical Interactions Between PriA and PriB in DNA Replication Restart Pathways in Neisseria Gonorrhoeae
title_sort functional consequences of physical interactions between pria and prib in dna replication restart pathways in neisseria gonorrhoeae
publisher University of Dayton / OhioLINK
publishDate 2011
url http://rave.ohiolink.edu/etdc/view?acc_num=dayton1323795871
work_keys_str_mv AT fengcui functionalconsequencesofphysicalinteractionsbetweenpriaandpribindnareplicationrestartpathwaysinneisseriagonorrhoeae
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