DEVELOPMENT OF LC-MS/MS ASSAYS FOR URINE DRUG TESTING AND QUANTIFICATION OF HUMAN STOOL HEMOGLOBIN FOR COLORECTAL CANCER SCREENING

Bibliographic Details
Main Author:	Alagandula, Ravali
Language:	English
Published:	Cleveland State University / OhioLINK 2017
Subjects:	Chemistry
Online Access:	http://rave.ohiolink.edu/etdc/view?acc_num=csu1513598216213184

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spelling	ndltd-OhioLink-oai-etd.ohiolink.edu-csu15135982162131842021-08-03T07:05:08Z DEVELOPMENT OF LC-MS/MS ASSAYS FOR URINE DRUG TESTING AND QUANTIFICATION OF HUMAN STOOL HEMOGLOBIN FOR COLORECTAL CANCER SCREENING Alagandula, Ravali Chemistry Mass Spectrometry allows the accurate determination and quantitation of drugs and proteins with high sensitivity and specificity, making it a powerful method to provide insights into various diseases using biological samples. My dissertation research focuses on the development of mass spectrometry-based methods to quantitate drugs and protein biomarkers in clinical specimen. The background on the development of robust mass spectrometry-based methods to quantify drugs and proteins in biological samples and various sample preparation techniques are described in Chapter I.My dissertation research is composed of two parts. In the first part, I developed three mass spectrometric methods to quantitate several important drugs related to pain management and drug abuse, solving the low-throughput problem encountered in clinical labs. Chapter II discusses the method developed for quantitating phenobarbital (a pain management drug) in human urine. Chapter III describes the method developed for quantitate ethyl-glucuronide (an alcohol biomarker) in human urine. In Chapter IV, I describe a method for quantification of phenobarbital and ethyl-glucuronide in urine using one MS/MS method. We have demonstrated that these methods developed have a throughput of ~700 samples/per day, much higher than the throughput of current methods. In the second part, my research focused on the development and application of a novel immunocapture-MRM method for quantitation of protein biomarkers in stool. Immunocapture-MRM combines immunoassays with mass spectrometry, taking advantages of targeted enrichment of protein biomarkers from complex clinical specimen using immunoassay and specific quantitation by mass spectrometry. Currently, less quantitative elution and digestion of the proteins captured by immunoassay have been a major problem associated with immunocapture-MRM based methods. The part of my research was devoted to the development of methods to solve this problem. Chapter V describes new methods I developed, which enable the quantitative elution and digestion of proteins captured by immunoassay. In Chapter VI, I discuss the development of an immunocapture-MRM method for quantitation of stool proteins and application of this assay to analyze stool samples. 2017-12-20 English text Cleveland State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=csu1513598216213184 http://rave.ohiolink.edu/etdc/view?acc_num=csu1513598216213184 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection	NDLTD
language	English
sources	NDLTD
topic	Chemistry
spellingShingle	Chemistry Alagandula, Ravali DEVELOPMENT OF LC-MS/MS ASSAYS FOR URINE DRUG TESTING AND QUANTIFICATION OF HUMAN STOOL HEMOGLOBIN FOR COLORECTAL CANCER SCREENING
author	Alagandula, Ravali
author_facet	Alagandula, Ravali
author_sort	Alagandula, Ravali
title	DEVELOPMENT OF LC-MS/MS ASSAYS FOR URINE DRUG TESTING AND QUANTIFICATION OF HUMAN STOOL HEMOGLOBIN FOR COLORECTAL CANCER SCREENING
title_short	DEVELOPMENT OF LC-MS/MS ASSAYS FOR URINE DRUG TESTING AND QUANTIFICATION OF HUMAN STOOL HEMOGLOBIN FOR COLORECTAL CANCER SCREENING
title_full	DEVELOPMENT OF LC-MS/MS ASSAYS FOR URINE DRUG TESTING AND QUANTIFICATION OF HUMAN STOOL HEMOGLOBIN FOR COLORECTAL CANCER SCREENING
title_fullStr	DEVELOPMENT OF LC-MS/MS ASSAYS FOR URINE DRUG TESTING AND QUANTIFICATION OF HUMAN STOOL HEMOGLOBIN FOR COLORECTAL CANCER SCREENING
title_full_unstemmed	DEVELOPMENT OF LC-MS/MS ASSAYS FOR URINE DRUG TESTING AND QUANTIFICATION OF HUMAN STOOL HEMOGLOBIN FOR COLORECTAL CANCER SCREENING
title_sort	development of lc-ms/ms assays for urine drug testing and quantification of human stool hemoglobin for colorectal cancer screening
publisher	Cleveland State University / OhioLINK
publishDate	2017
url	http://rave.ohiolink.edu/etdc/view?acc_num=csu1513598216213184
work_keys_str_mv	AT alagandularavali developmentoflcmsmsassaysforurinedrugtestingandquantificationofhumanstoolhemoglobinforcolorectalcancerscreening
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DEVELOPMENT OF LC-MS/MS ASSAYS FOR URINE DRUG TESTING AND QUANTIFICATION OF HUMAN STOOL HEMOGLOBIN FOR COLORECTAL CANCER SCREENING

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