Age and Sex-Specific Effect of Caloric Restriction on Circadian Clock and Longevity-Associated Gene Expression

Age and Sex-Specific Effect of Caloric Restriction on Circadian Clock and Longevity-Associated Gene Expression

Bibliographic Details
Main Author: Astafev, Artem Andreyevich
Language:English
Published: Cleveland State University / OhioLINK 2017
Subjects:
Biology
Molecular Biology
caloric restriction
calorie restriction
CR
circadian clock
circadian rhythms
aging
sexual dimorphism
sex-specific
sex-dependent
short-term CR
long-term CR
dietary restriction
diet
longevity
gene expression
metabolism
feeding regimen
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=csu1508436079666819
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-csu15084360796668192021-08-03T07:04:23Z Age and Sex-Specific Effect of Caloric Restriction on Circadian Clock and Longevity-Associated Gene Expression Astafev, Artem Andreyevich Biology Molecular Biology caloric restriction calorie restriction CR circadian clock circadian rhythms aging sexual dimorphism sex-specific sex-dependent short-term CR long-term CR dietary restriction diet longevity gene expression metabolism feeding regimen <p>The rhythms in the expression of circadian clock genes are affected by calorie restriction (CR), a dietary paradigm known to increase lifespan. In our current study, we show that circadian rhythms are influenced by sex and the effects of CR are different between males and females. In particular, we found a group of clock genes which showed a sex-dependent difference in expression, as well as in response to CR (<i>Rev-Erb α</i>, <i>Ror γ</i> and both Cryptochromes: <i>Cry1</i> and <i>Cry2</i> genes). Two clock genes showed no difference in expression but their response to CR showed sexual dimorphism (<i>Ror α</i> and <i>Rev-Erb β</i>). Finally, we found some of the clock genes to be expressed in a sex-independent manner (<i>Bmal1</i>, <i>Per1</i>, <i>Per2</i> and <i>Per3</i>). The response to CR for these genes did not show sexual dimorphism as well. Several genes were also previously reported to be regulated by CR. These genes showed a sex-dependent difference in expression as well as the sexual dimorphism in the response to CR in mouse liver (<i>Fmo3</i>, <i>Mup4</i>, <i>Serpina12</i>, <i>Cyp4a12b</i> and <i>Cyp4a14a</i>). IGF signaling plays an important role in aging and CR effects. <i>Igf-1</i> expression is regulated by CR and by the circadian clock, we found that rhythms in <i>Igf-1</i> expression have sexual dimorphism.</p><p>We also investigated the differences in expression levels in young versus old mice to see if the effect of short-term CR differs from the effect of long-term CR. In all of the 4 analyzed genes, the expression does not change with age. The effect of CR on the expression of 2 out of 4 genes (<i>Bmal1</i> and <i>Per2</i>) was lost upon long-term exposure to CR. For the other 2 genes (<i>Per1</i> and <i>Per3</i>) the effect of CR persisted over time. Thus we emphasize that sex and age are important factors for consideration when administering CR.</p> 2017-10-20 English text Cleveland State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=csu1508436079666819 http://rave.ohiolink.edu/etdc/view?acc_num=csu1508436079666819 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Biology
Molecular Biology
caloric restriction
calorie restriction
CR
circadian clock
circadian rhythms
aging
sexual dimorphism
sex-specific
sex-dependent
short-term CR
long-term CR
dietary restriction
diet
longevity
gene expression
metabolism
feeding regimen
spellingShingle Biology
Molecular Biology
caloric restriction
calorie restriction
CR
circadian clock
circadian rhythms
aging
sexual dimorphism
sex-specific
sex-dependent
short-term CR
long-term CR
dietary restriction
diet
longevity
gene expression
metabolism
feeding regimen
Astafev, Artem Andreyevich
Age and Sex-Specific Effect of Caloric Restriction on Circadian Clock and Longevity-Associated Gene Expression
author Astafev, Artem Andreyevich
author_facet Astafev, Artem Andreyevich
author_sort Astafev, Artem Andreyevich
title Age and Sex-Specific Effect of Caloric Restriction on Circadian Clock and Longevity-Associated Gene Expression
title_short Age and Sex-Specific Effect of Caloric Restriction on Circadian Clock and Longevity-Associated Gene Expression
title_full Age and Sex-Specific Effect of Caloric Restriction on Circadian Clock and Longevity-Associated Gene Expression
title_fullStr Age and Sex-Specific Effect of Caloric Restriction on Circadian Clock and Longevity-Associated Gene Expression
title_full_unstemmed Age and Sex-Specific Effect of Caloric Restriction on Circadian Clock and Longevity-Associated Gene Expression
title_sort age and sex-specific effect of caloric restriction on circadian clock and longevity-associated gene expression
publisher Cleveland State University / OhioLINK
publishDate 2017
url http://rave.ohiolink.edu/etdc/view?acc_num=csu1508436079666819
work_keys_str_mv AT astafevartemandreyevich ageandsexspecificeffectofcaloricrestrictiononcircadianclockandlongevityassociatedgeneexpression
_version_ 1719452679427588096

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