SALINE ADAPTATION OF THE MICROALGA Scenedesmus dimorphus FROM FRESH WATER TO BRACKISH WATER
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Cleveland State University / OhioLINK
2013
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=csu1382355969 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-csu13823559692021-08-03T06:19:53Z SALINE ADAPTATION OF THE MICROALGA Scenedesmus dimorphus FROM FRESH WATER TO BRACKISH WATER Gigante, Bethany Marie Botany Environmental Science Experiments Sustainability Biology Biochemistry Scenedesmus dimorphus salt stress lipid saltwater adaptation brackish water adaptation algal adaptation freshwater algae algal biomass semi-batch closed system open system growth rates contamination The fresh water microalga Scenedesmus dimorphus is of interest in the microalgae to biofuel field. With a 26% dry weight as lipid content and a potential doubling time of 60-70 hours, it is a viable candidate for biofuel production. Recent studies have focused on optimizing the growth rate and lipid content of S. dimorphus by finding ideal light, carbon-dioxide flow rates, nutrient composition of media, pH, temperature, and implementation of various stresses in the growth environment. Adaptation of S. dimorphus to a saline environment would make it a viable biofuel stock world-wide. This saline adaptation experiment was conducted in two stages, using TSG as a unit of measurement for salt density of the growth environments. The goal for the first stage of this study was to determine if S. dimorphus was capable of adapting to increased salinity levels in a closed environment, where competition with other algal species and bacteria were limited to non-existent. The second stage of experimentation determined if the adapted S. dimorphus could survive in a large scale open, marine system. Adaptation of S. dimorphus from fresh water to brackish water was successfully completed after a 220 day semi-batch process. Adaptation to brackish water environments yielded no significant difference in growth rates when compared to fresh water environments. Adapted S. dimorphus obtained a growth rate of 0.47 ±0.14 day-1 in a 1.015 TSG small-scale, closed system environment, while the freshwater control obtained a growth rate of 0.56 ±0.05 day-1. Adapted S. dimorphus obtained a growth rate of 0.35 ±0.15 day-1 in a 1.015 TSG large-scale, open system environment, which was not statistically different from that of freshwater S. dimorphus in freshwater media, with growth rate of 0.61 ±0.31 day-1. Lipid content in the 1.015 TSG small-scale, closed system was calculated to be 23 ±10%, with the large scale, open system yielding lipid content results of 15±9%. The microalga S. dimorphus is capable of adaptation to brackish water environments with slight, but insignificant decreases in growth rate and lipid content. 2013-10-24 English text Cleveland State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=csu1382355969 http://rave.ohiolink.edu/etdc/view?acc_num=csu1382355969 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
| collection |
NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Botany Environmental Science Experiments Sustainability Biology Biochemistry Scenedesmus dimorphus salt stress lipid saltwater adaptation brackish water adaptation algal adaptation freshwater algae algal biomass semi-batch closed system open system growth rates contamination |
| spellingShingle |
Botany Environmental Science Experiments Sustainability Biology Biochemistry Scenedesmus dimorphus salt stress lipid saltwater adaptation brackish water adaptation algal adaptation freshwater algae algal biomass semi-batch closed system open system growth rates contamination Gigante, Bethany Marie SALINE ADAPTATION OF THE MICROALGA Scenedesmus dimorphus FROM FRESH WATER TO BRACKISH WATER |
| author |
Gigante, Bethany Marie |
| author_facet |
Gigante, Bethany Marie |
| author_sort |
Gigante, Bethany Marie |
| title |
SALINE ADAPTATION OF THE MICROALGA Scenedesmus dimorphus FROM FRESH WATER TO BRACKISH WATER |
| title_short |
SALINE ADAPTATION OF THE MICROALGA Scenedesmus dimorphus FROM FRESH WATER TO BRACKISH WATER |
| title_full |
SALINE ADAPTATION OF THE MICROALGA Scenedesmus dimorphus FROM FRESH WATER TO BRACKISH WATER |
| title_fullStr |
SALINE ADAPTATION OF THE MICROALGA Scenedesmus dimorphus FROM FRESH WATER TO BRACKISH WATER |
| title_full_unstemmed |
SALINE ADAPTATION OF THE MICROALGA Scenedesmus dimorphus FROM FRESH WATER TO BRACKISH WATER |
| title_sort |
saline adaptation of the microalga scenedesmus dimorphus from fresh water to brackish water |
| publisher |
Cleveland State University / OhioLINK |
| publishDate |
2013 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=csu1382355969 |
| work_keys_str_mv |
AT gigantebethanymarie salineadaptationofthemicroalgascenedesmusdimorphusfromfreshwatertobrackishwater |
| _version_ |
1719434768474439680 |