DESIGN, SYNTHESIS AND CHARACTERIZATION OF CHAIN-END FUNCTIONALIZED GLYCO-POLYMER FOR EFFICIENT PROTEOMIC ANALYSIS

Bibliographic Details
Main Author:	Satya , Narla Nandana
Language:	English
Published:	Cleveland State University / OhioLINK 2013
Subjects:	Biochemistry
Online Access:	http://rave.ohiolink.edu/etdc/view?acc_num=csu1372333847

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spelling	ndltd-OhioLink-oai-etd.ohiolink.edu-csu13723338472021-08-03T05:24:22Z DESIGN, SYNTHESIS AND CHARACTERIZATION OF CHAIN-END FUNCTIONALIZED GLYCO-POLYMER FOR EFFICIENT PROTEOMIC ANALYSIS Satya , Narla Nandana Biochemistry Carbohydrates, especially, cell surface carbohydrates act as receptors for a variety of ligands such as proteins and thereby play significant roles in physiological and pathological processes, including cellular recognition and communication, bacterial and viral infection, and tumor metastasis. As such, binding interactions of carbohydrates and proteins have provided a starting point for the development of concept of isolation and probing of proteins and cells in biological research and novel diagnostic agents as well as new therapeutic applications. In this thesis study, oriented and multivalent carbohydrate macromolecules were designed and developed based on a chain-end functionalized carbohydrate-containing polymer (glycopolymer) for efficient protein analysis. Firstly, O-cyanate chain-end functionalized glycopolymer was synthesized via cyanoxyl-mediated free-radical polymerization (CMFRP) in one-pot fashion. This glycopolymer was tested for its oriented glyco-marcroligand formation and its glyco-affinity capturing and glycanarray application by combining O-cyanate chain-end functionalized glycopolymer with commercially available amine-functionalized silica gel and glass slide via isourea bond formation, respectively. In the second study we demonstrated a new type of glycan microarray, namely, oriented and density controlled glyco-macroligand microarray based on end-point immobilization of glycopolymer that was accompanied with boronic acid (BA) ligands in different sizes as detachable “temporary molecular spacers”. The spaced glycopolymer microarray showed enhanced lectin binding compared to non-spaced one. Third, a chemoenzymatic synthesis of chain-end functionalized sialyllactose-containing glycopolymers with different linkages was performed and their oriented immobilization for glycoarray and SPR-based glyco-biosensor applications were studied. Specifically, O-cyanate chain-end functionalized sialyllactose-containing glycopolymers were synthesized by enzymatic a2,3- and a2,6- sialylation of a lactose-containing glycopolymer and characterized by 1H NMR. They were printed onto amine-functionalized glass slides for glycoarray formation. Specific protein binding activity of the arrays were confirmed with a2,3- and a2,6-sialyl specific binding lectins together with inhibition assays. In addition, sialyllactose glycopolymers were immobilized on amine-modified SPR chip to study the specific binding activity of lectins and influenza viral hemagglutinins (HA). These sialyloligo-macroligand derivatives combined with oriental and density controlled glycan array and SPR-based glyco-biosensor are closely to mimic 3D nature presentation of sialyloligosaccharides and will provide important high-throughput tools for virus diagnosis and potential anti-viral drug candidates screening application. 2013-07-08 English text Cleveland State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=csu1372333847 http://rave.ohiolink.edu/etdc/view?acc_num=csu1372333847 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection	NDLTD
language	English
sources	NDLTD
topic	Biochemistry
spellingShingle	Biochemistry Satya , Narla Nandana DESIGN, SYNTHESIS AND CHARACTERIZATION OF CHAIN-END FUNCTIONALIZED GLYCO-POLYMER FOR EFFICIENT PROTEOMIC ANALYSIS
author	Satya , Narla Nandana
author_facet	Satya , Narla Nandana
author_sort	Satya , Narla Nandana
title	DESIGN, SYNTHESIS AND CHARACTERIZATION OF CHAIN-END FUNCTIONALIZED GLYCO-POLYMER FOR EFFICIENT PROTEOMIC ANALYSIS
title_short	DESIGN, SYNTHESIS AND CHARACTERIZATION OF CHAIN-END FUNCTIONALIZED GLYCO-POLYMER FOR EFFICIENT PROTEOMIC ANALYSIS
title_full	DESIGN, SYNTHESIS AND CHARACTERIZATION OF CHAIN-END FUNCTIONALIZED GLYCO-POLYMER FOR EFFICIENT PROTEOMIC ANALYSIS
title_fullStr	DESIGN, SYNTHESIS AND CHARACTERIZATION OF CHAIN-END FUNCTIONALIZED GLYCO-POLYMER FOR EFFICIENT PROTEOMIC ANALYSIS
title_full_unstemmed	DESIGN, SYNTHESIS AND CHARACTERIZATION OF CHAIN-END FUNCTIONALIZED GLYCO-POLYMER FOR EFFICIENT PROTEOMIC ANALYSIS
title_sort	design, synthesis and characterization of chain-end functionalized glyco-polymer for efficient proteomic analysis
publisher	Cleveland State University / OhioLINK
publishDate	2013
url	http://rave.ohiolink.edu/etdc/view?acc_num=csu1372333847
work_keys_str_mv	AT satyanarlanandana designsynthesisandcharacterizationofchainendfunctionalizedglycopolymerforefficientproteomicanalysis
_version_	1719419731830636544

DESIGN, SYNTHESIS AND CHARACTERIZATION OF CHAIN-END FUNCTIONALIZED GLYCO-POLYMER FOR EFFICIENT PROTEOMIC ANALYSIS

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