Ellagic Acid-Mediated CK2 Inhibition, A Natural, Multifunctional Strategy to Trigger Cervical Cancer Cell Death in Vitro and in Vivo

Bibliographic Details
Main Author:	Mohammad, Hanan Fathi
Language:	English
Published:	Cleveland State University / OhioLINK 2012
Subjects:	Biochemistry Biology Cellular Biology Health Care Molecular Biology Oncology Virology Ellagic acid CK2 Cervical Cancer HPV IAP's
Online Access:	http://rave.ohiolink.edu/etdc/view?acc_num=csu1337789869

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spelling	ndltd-OhioLink-oai-etd.ohiolink.edu-csu13377898692021-08-03T05:35:19Z Ellagic Acid-Mediated CK2 Inhibition, A Natural, Multifunctional Strategy to Trigger Cervical Cancer Cell Death in Vitro and in Vivo Mohammad, Hanan Fathi Biochemistry Biology Cellular Biology Health Care Molecular Biology Oncology Virology Ellagic acid CK2 Cervical Cancer HPV IAP's Targeting CK2 for cancer therapy has proven effective in inhibiting tumor growth in several histology’s. The main goal of this study was to acquire pre-clinical data on a naturally occurring CK2-inhibitor (ellagic acid) in support of a human clinical trial. In vitro analysis included testing the spectrum of ellagic acid anti-cancer activity, defining the mode of growth inhibition in cancer cells and its impact on biochemical and molecular pathways. In vivo studies included determination of ellagic acid inhibitory effect in animal tumor models and toxicity analysis. We found that CK2 inhibition mediated by ellagic acid, was effective in inhibiting the growth of a wide spectrum of human cancer cells. These include breast cancer (MCF-7), kidney cancer (ACHN), colon adenocarcinoma (SW480), glioblastoma (U87), prostate cancer (DU-145), melanoma (WM164) and HPV -positive (HeLa) and -negative (C33A) cervical carcinomas. We used HeLa and C33A cell lines to determine EA mechanisms of action and impact on oncogenic, proliferative, and apoptotic pathways. Since CK2 controls over 300 target proteins involved in major biochemical and molecular pathways, we found that ellagic acid treatment was able to inhibit HPV oncogene expression, interrupt oncogenic pathways and induce anti-proliferative and pro-apoptotic pathways in HeLa cells. In C33A cells, CK2 inhibition bypassed tumor suppressor p53 and pRb mutation and induced apoptosis by inhibition of anti-apoptotic proteins IAPs. Comparing HeLa and C33A cells response to ellagic acid treatment, C33A cells were more sensitive to ellagic acid treatment compared to HeLa. The expression of HPV oncogenes in HeLa cells could delay HeLa response to death stimuli exerted by CK2 inhibition. CK2 inhibition and subsequent IAPs repression is the common event occurring in HeLa and C33A cells. 2012-05-23 English text Cleveland State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=csu1337789869 http://rave.ohiolink.edu/etdc/view?acc_num=csu1337789869 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection	NDLTD
language	English
sources	NDLTD
topic	Biochemistry Biology Cellular Biology Health Care Molecular Biology Oncology Virology Ellagic acid CK2 Cervical Cancer HPV IAP's
spellingShingle	Biochemistry Biology Cellular Biology Health Care Molecular Biology Oncology Virology Ellagic acid CK2 Cervical Cancer HPV IAP's Mohammad, Hanan Fathi Ellagic Acid-Mediated CK2 Inhibition, A Natural, Multifunctional Strategy to Trigger Cervical Cancer Cell Death in Vitro and in Vivo
author	Mohammad, Hanan Fathi
author_facet	Mohammad, Hanan Fathi
author_sort	Mohammad, Hanan Fathi
title	Ellagic Acid-Mediated CK2 Inhibition, A Natural, Multifunctional Strategy to Trigger Cervical Cancer Cell Death in Vitro and in Vivo
title_short	Ellagic Acid-Mediated CK2 Inhibition, A Natural, Multifunctional Strategy to Trigger Cervical Cancer Cell Death in Vitro and in Vivo
title_full	Ellagic Acid-Mediated CK2 Inhibition, A Natural, Multifunctional Strategy to Trigger Cervical Cancer Cell Death in Vitro and in Vivo
title_fullStr	Ellagic Acid-Mediated CK2 Inhibition, A Natural, Multifunctional Strategy to Trigger Cervical Cancer Cell Death in Vitro and in Vivo
title_full_unstemmed	Ellagic Acid-Mediated CK2 Inhibition, A Natural, Multifunctional Strategy to Trigger Cervical Cancer Cell Death in Vitro and in Vivo
title_sort	ellagic acid-mediated ck2 inhibition, a natural, multifunctional strategy to trigger cervical cancer cell death in vitro and in vivo
publisher	Cleveland State University / OhioLINK
publishDate	2012
url	http://rave.ohiolink.edu/etdc/view?acc_num=csu1337789869
work_keys_str_mv	AT mohammadhananfathi ellagicacidmediatedck2inhibitionanaturalmultifunctionalstrategytotriggercervicalcancercelldeathinvitroandinvivo
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Ellagic Acid-Mediated CK2 Inhibition, A Natural, Multifunctional Strategy to Trigger Cervical Cancer Cell Death in Vitro and in Vivo

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