CapAT "An adipose-enriched isoform"
| Main Author: | |
|---|---|
| Language: | English |
| Published: |
Case Western Reserve University School of Graduate Studies / OhioLINK
2021
|
| Subjects: | |
| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=case1619710218494047 |
| id |
ndltd-OhioLink-oai-etd.ohiolink.edu-case1619710218494047 |
|---|---|
| record_format |
oai_dc |
| spelling |
ndltd-OhioLink-oai-etd.ohiolink.edu-case16197102184940472021-08-03T07:17:18Z CapAT "An adipose-enriched isoform" He, Yue Molecular Biology Insulin adipose tissue glucose uptake IL-6 Adipose tissue is an insulin-sensitive organ, and many cellular processes in adipocytes are activated by insulin stimulation. Glucose uptake is one of the critical metabolisms stimulated by insulin, which is mainly driven by glucose transporter 4 (GLUT4). Previous studiese have identified two pathways that are needed for GLUT4 translocation, one of which is the Cbl/CAP pathway. The main focus of this study is the substrate Cbl-associated protein (CAP) in the Cbl/CAP pathway. In order to study the function of the CAP, I used siRNA and plasmids to knockdown or overexpress it, and then implemented the glucose uptake assay to figure out how CAP influenced glucose uptake. My results suggest that CAP didn’t have a significant influence on glucose uptake. By measuring the mRNA level of IL-6 after knockdown and overexpression, I also tested the relationship between CAP and inflammation in fat cells, but the results of this experiment didn’t give a clear answer on their connections. 2021-06-21 English text Case Western Reserve University School of Graduate Studies / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=case1619710218494047 http://rave.ohiolink.edu/etdc/view?acc_num=case1619710218494047 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
| collection |
NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Molecular Biology Insulin adipose tissue glucose uptake IL-6 |
| spellingShingle |
Molecular Biology Insulin adipose tissue glucose uptake IL-6 He, Yue CapAT "An adipose-enriched isoform" |
| author |
He, Yue |
| author_facet |
He, Yue |
| author_sort |
He, Yue |
| title |
CapAT "An adipose-enriched isoform" |
| title_short |
CapAT "An adipose-enriched isoform" |
| title_full |
CapAT "An adipose-enriched isoform" |
| title_fullStr |
CapAT "An adipose-enriched isoform" |
| title_full_unstemmed |
CapAT "An adipose-enriched isoform" |
| title_sort |
capat "an adipose-enriched isoform" |
| publisher |
Case Western Reserve University School of Graduate Studies / OhioLINK |
| publishDate |
2021 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=case1619710218494047 |
| work_keys_str_mv |
AT heyue capatanadiposeenrichedisoform |
| _version_ |
1719458282337206272 |