Towards Development of Affinity Polymer-Based Adhesion Barriers for Surgical Mesh Devices
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Case Western Reserve University School of Graduate Studies / OhioLINK
2021
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=case1612871430445022 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-case16128714304450222021-08-03T07:16:40Z Towards Development of Affinity Polymer-Based Adhesion Barriers for Surgical Mesh Devices Learn, Greg Daniel Biomedical Engineering Adhesion Barrier Adhesion Prevention Adhesions Biofouling Biomaterial Biotextile Cyclodextrin Hernia Hernia Mesh Implant Mesh Peritoneal Adhesions Plasma Polymer Polypropylene Post-Surgical Adhesions Surgery Surgical Mesh Textile Post-surgical adhesions are internal scars that pathologically adhere together adjacent tissues/organs/biomaterials. They pose a tremendous but frequently underestimated burden across many surgical disciplines, being especially prevalent following abdominal surgery. Peritoneal adhesions can cause discomfort, intestinal obstructions, infertility, and increased morbidity/mortality of subsequent surgery. Once formed, treatments for adhesions tend to be risky and ineffective, so prophylactic strategies are desirable. Implantation of meshes, such as in hernia repair, often exacerbates peritoneal adhesions. Knitted polypropylene (PP) meshes are the most common hernioplasty devices, but are notoriously adhesiogenic owing to material and structural characteristics that promote incorporation, such as hydrophobicity and reticular construction. The ideal strategy to prevent mesh adhesions entails adhering a smooth, continuous, hydrophilic barrier material on the mesh visceral face to mitigate tissue attachment processes. Prior studies developed polymerized cyclodextrin (pCD) materials having unique capabilities for sustained, multi-window drug release, and suggested that these hydrophilic polymers passively resist cell attachment. In several animal species, pCD could deliver antibiotics for weeks to successfully resolve mesh infection, another hernioplasty complication for which only suboptimal solutions exist. In the present work, pCD materials were explored toward application as novel adhesion barriers for PP surgical meshes. First, nonthermal plasma activation was assessed as a strategy to improve PP-pCD bonding, as PP is generally unreceptive to coatings. Plasma introduced hydroxyls onto PP, enhancing PP-pCD adherence. Second, protein adsorption, bacterial attachment, and fibroblast viability/attachment upon pCD-coated and bare PP materials were evaluated. These events play roles in mesh adhesion, infection, and biocompatibility. pCD decreased protein adsorption and bacterial attachment to PP, without fibroblast cytotoxicity. Third, effects of PP plasma activation on protein adsorption, fibroblast/bacterial attachment, and mesh mechanical properties were investigated. Regardless of duration, plasma exposure of bare PP reduced protein adsorption and bacterial attachment, and increased fibroblast attachment, but longer treatments progressively embrittled PP mesh. Fourth, preliminary studies in vivo explored effects of pCD barriers on adhesions to PP mesh. These animal experiments suggested that pCD-covered mesh surfaces resisted adhesions, while bare PP meshes did not. Altogether, pCD materials have potential as adhesion barriers that could uniquely combat both mesh adhesions and prosthetic infection. 2021-06-21 English text Case Western Reserve University School of Graduate Studies / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=case1612871430445022 http://rave.ohiolink.edu/etdc/view?acc_num=case1612871430445022 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
| collection |
NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Biomedical Engineering Adhesion Barrier Adhesion Prevention Adhesions Biofouling Biomaterial Biotextile Cyclodextrin Hernia Hernia Mesh Implant Mesh Peritoneal Adhesions Plasma Polymer Polypropylene Post-Surgical Adhesions Surgery Surgical Mesh Textile |
| spellingShingle |
Biomedical Engineering Adhesion Barrier Adhesion Prevention Adhesions Biofouling Biomaterial Biotextile Cyclodextrin Hernia Hernia Mesh Implant Mesh Peritoneal Adhesions Plasma Polymer Polypropylene Post-Surgical Adhesions Surgery Surgical Mesh Textile Learn, Greg Daniel Towards Development of Affinity Polymer-Based Adhesion Barriers for Surgical Mesh Devices |
| author |
Learn, Greg Daniel |
| author_facet |
Learn, Greg Daniel |
| author_sort |
Learn, Greg Daniel |
| title |
Towards Development of Affinity Polymer-Based Adhesion Barriers for Surgical Mesh Devices |
| title_short |
Towards Development of Affinity Polymer-Based Adhesion Barriers for Surgical Mesh Devices |
| title_full |
Towards Development of Affinity Polymer-Based Adhesion Barriers for Surgical Mesh Devices |
| title_fullStr |
Towards Development of Affinity Polymer-Based Adhesion Barriers for Surgical Mesh Devices |
| title_full_unstemmed |
Towards Development of Affinity Polymer-Based Adhesion Barriers for Surgical Mesh Devices |
| title_sort |
towards development of affinity polymer-based adhesion barriers for surgical mesh devices |
| publisher |
Case Western Reserve University School of Graduate Studies / OhioLINK |
| publishDate |
2021 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=case1612871430445022 |
| work_keys_str_mv |
AT learngregdaniel towardsdevelopmentofaffinitypolymerbasedadhesionbarriersforsurgicalmeshdevices |
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1719457915311489024 |