Tubular Tissue Engineered Scaffold-Free High-Cell-Density Mesenchymal Condensations For Femoral Defect Regeneration

Tubular Tissue Engineered Scaffold-Free High-Cell-Density Mesenchymal Condensations For Femoral Defect Regeneration

Bibliographic Details
Main Author: Varghai, Daniel
Language:English
Published: Case Western Reserve University School of Graduate Studies / OhioLINK 2017
Subjects:
Biomedical Engineering
Biology
Biomedical Research
Cellular Biology
Engineering
Health Sciences
Histology
Medical Imaging
Microbiology
Morphology
Growth factors
TGF-B1
BMP-2
controlled delivery
microparticles
chondrogenesis
osteogenesis
mold
self-assembly
regenerative medicine
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=case1497222797338966
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-case14972227973389662021-08-03T07:02:54Z Tubular Tissue Engineered Scaffold-Free High-Cell-Density Mesenchymal Condensations For Femoral Defect Regeneration Varghai, Daniel Biomedical Engineering Biology Biomedical Research Cellular Biology Engineering Health Sciences Histology Medical Imaging Microbiology Morphology Growth factors TGF-B1 BMP-2 controlled delivery microparticles chondrogenesis osteogenesis mold self-assembly regenerative medicine Lower-limb fracture exhibits a limited capacity to heal critically sized defects and biomimetic tissue engineering is a promising approach for addressing this clinical need. Here, scaffold-free tubular mesenchymal condensations featuring temporally controlled TGF-Β1 and BMP-2 morphogen release from incorporated microparticles were engineered to form self-assembled rings and tubes. In vitro culture, subcutaneous, and femoral defect implantation was performed to establish bone forming capacity with the treatment groups: 1) TGF-Β1, 2) BMP-2, or 3) BMP-2+TGF-Β1. Bone formation was evaluated by biochemical, μCT, and histological analyses. Dual-delivery enhanced bone volume versus single morphogens at 6wks, and histology revealed bone within tubular geometries with enhanced cartilage, mimetic of endochondral ossification. Overall: 1) geometry of scaffold-free mesenchymal condensations guided 3D ossification, 2) BMP-2+TGF-Β1 presentation augmented ectopic bone formation, and 3) tubular architecture promoted femoral defect bridging over random organization. 2017-08-30 English text Case Western Reserve University School of Graduate Studies / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=case1497222797338966 http://rave.ohiolink.edu/etdc/view?acc_num=case1497222797338966 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Biomedical Engineering
Biology
Biomedical Research
Cellular Biology
Engineering
Health Sciences
Histology
Medical Imaging
Microbiology
Morphology
Growth factors
TGF-B1
BMP-2
controlled delivery
microparticles
chondrogenesis
osteogenesis
mold
self-assembly
regenerative medicine
spellingShingle Biomedical Engineering
Biology
Biomedical Research
Cellular Biology
Engineering
Health Sciences
Histology
Medical Imaging
Microbiology
Morphology
Growth factors
TGF-B1
BMP-2
controlled delivery
microparticles
chondrogenesis
osteogenesis
mold
self-assembly
regenerative medicine
Varghai, Daniel
Tubular Tissue Engineered Scaffold-Free High-Cell-Density Mesenchymal Condensations For Femoral Defect Regeneration
author Varghai, Daniel
author_facet Varghai, Daniel
author_sort Varghai, Daniel
title Tubular Tissue Engineered Scaffold-Free High-Cell-Density Mesenchymal Condensations For Femoral Defect Regeneration
title_short Tubular Tissue Engineered Scaffold-Free High-Cell-Density Mesenchymal Condensations For Femoral Defect Regeneration
title_full Tubular Tissue Engineered Scaffold-Free High-Cell-Density Mesenchymal Condensations For Femoral Defect Regeneration
title_fullStr Tubular Tissue Engineered Scaffold-Free High-Cell-Density Mesenchymal Condensations For Femoral Defect Regeneration
title_full_unstemmed Tubular Tissue Engineered Scaffold-Free High-Cell-Density Mesenchymal Condensations For Femoral Defect Regeneration
title_sort tubular tissue engineered scaffold-free high-cell-density mesenchymal condensations for femoral defect regeneration
publisher Case Western Reserve University School of Graduate Studies / OhioLINK
publishDate 2017
url http://rave.ohiolink.edu/etdc/view?acc_num=case1497222797338966
work_keys_str_mv AT varghaidaniel tubulartissueengineeredscaffoldfreehighcelldensitymesenchymalcondensationsforfemoraldefectregeneration
_version_ 1719452351486492672

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