TRACKING AN ELECTRICALLY SILENT SOURCE IN THE HIPPOCAMPUS USING A CALCIUM-SENSITIVE FLUORESCENT DYE

Bibliographic Details
Main Author:	Keshav, Arvind
Language:	English
Published:	Case Western Reserve University School of Graduate Studies / OhioLINK 2016
Subjects:	Biomedical Engineering Biomedical Research epilepsy electrically silent source calcium-sensitive fluorescent dyes NMDA
Online Access:	http://rave.ohiolink.edu/etdc/view?acc_num=case1469380261

id	ndltd-OhioLink-oai-etd.ohiolink.edu-case1469380261
record_format	oai_dc
spelling	ndltd-OhioLink-oai-etd.ohiolink.edu-case14693802612021-08-03T06:37:49Z TRACKING AN ELECTRICALLY SILENT SOURCE IN THE HIPPOCAMPUS USING A CALCIUM-SENSITIVE FLUORESCENT DYE Keshav, Arvind Biomedical Engineering Biomedical Research epilepsy electrically silent source calcium-sensitive fluorescent dyes NMDA Analysis of the neural activity in the hippocampus in the presence of epileptogenic agents shows fast-moving spikes sensitive to N-Methyl-D-Aspartate (NMDA) coming from a putative electrically silent source moving at a slower speed and difficult to track with standard fluorescence methods. Advances in imaging technology have allowed for millisecond-resolution mapping of the changes in fluorescence with high spatial resolution. In this study, the presence of this electrically silent focus was revealed by mapping the increase in intracellular calcium using the calcium-sensitive fluorescent dye Oregon Green 488 BAPTA-1 (OGB-1). Results indicate that the focus and its propagation can indeed be tracked using OGB-1, with a mean propagation velocity, obtained by cross-correlation calculations, of 0.0036 ± 0.0009 m/s, well within the predicted range for the putative source from other indirect measurements. Additionally, we tested in vitro the hypothesis that the propagation of this focus was independent of the NMDA-sensitive spikes it generates. The NMDA blocker introduced prevented the generation of spikes, but the movement of the focus was unaffected. The mean propagation velocity was 0.0035 ± 0.001 m/s and t-test results showed no significant difference in propagation speeds with and without the NMDA blocker. Together, these results indicate that the electrically silent focus is indeed the source of the spikes and relies on a different unknown mechanism of propagation. A possible mechanism of propagation is through the diffusion of potassium. However, the speed of the potassium wave was found to be significantly lower than that observed for our source (P<0.0001). These results indicate the presence of a novel calcium wave in the hippocampus propagating through pyramidal cells and capable of generating NMDA-sensitive spikes. 2016-09-13 English text Case Western Reserve University School of Graduate Studies / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=case1469380261 http://rave.ohiolink.edu/etdc/view?acc_num=case1469380261 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection	NDLTD
language	English
sources	NDLTD
topic	Biomedical Engineering Biomedical Research epilepsy electrically silent source calcium-sensitive fluorescent dyes NMDA
spellingShingle	Biomedical Engineering Biomedical Research epilepsy electrically silent source calcium-sensitive fluorescent dyes NMDA Keshav, Arvind TRACKING AN ELECTRICALLY SILENT SOURCE IN THE HIPPOCAMPUS USING A CALCIUM-SENSITIVE FLUORESCENT DYE
author	Keshav, Arvind
author_facet	Keshav, Arvind
author_sort	Keshav, Arvind
title	TRACKING AN ELECTRICALLY SILENT SOURCE IN THE HIPPOCAMPUS USING A CALCIUM-SENSITIVE FLUORESCENT DYE
title_short	TRACKING AN ELECTRICALLY SILENT SOURCE IN THE HIPPOCAMPUS USING A CALCIUM-SENSITIVE FLUORESCENT DYE
title_full	TRACKING AN ELECTRICALLY SILENT SOURCE IN THE HIPPOCAMPUS USING A CALCIUM-SENSITIVE FLUORESCENT DYE
title_fullStr	TRACKING AN ELECTRICALLY SILENT SOURCE IN THE HIPPOCAMPUS USING A CALCIUM-SENSITIVE FLUORESCENT DYE
title_full_unstemmed	TRACKING AN ELECTRICALLY SILENT SOURCE IN THE HIPPOCAMPUS USING A CALCIUM-SENSITIVE FLUORESCENT DYE
title_sort	tracking an electrically silent source in the hippocampus using a calcium-sensitive fluorescent dye
publisher	Case Western Reserve University School of Graduate Studies / OhioLINK
publishDate	2016
url	http://rave.ohiolink.edu/etdc/view?acc_num=case1469380261
work_keys_str_mv	AT keshavarvind trackinganelectricallysilentsourceinthehippocampususingacalciumsensitivefluorescentdye
_version_	1719440382904762368

TRACKING AN ELECTRICALLY SILENT SOURCE IN THE HIPPOCAMPUS USING A CALCIUM-SENSITIVE FLUORESCENT DYE

Similar Items