Genomic Analyses of the <i>Anopheles punctulatus</i> Group: Insights into Mosquito Biology and Implications for Vector Control and Disease Transmission
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Case Western Reserve University School of Graduate Studies / OhioLINK
2016
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=case1455900373 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-case14559003732021-08-03T06:34:51Z Genomic Analyses of the <i>Anopheles punctulatus</i> Group: Insights into Mosquito Biology and Implications for Vector Control and Disease Transmission Logue, Kyle J. Biology Genetics <p>Members of the <i>Anopheles punctulatus</i> (AP) group are the primary vectors of malaria and filariasis in Papua New Guinea. The AP group includes 13 morphologically similar sibling species (the <i>An. farauti</i> complex includes 8 identical species) that have overlapping geographic distributions. Currently, little is known about the biology of the AP group as few genetic markers are available and laboratory studies are difficult. Here, I utilized high-throughput sequencing methods to generate genetic data for five members of the AP group. I use this data to understand the divergence, extent of introgression and blood feeding patterns of <i>An. punctulatus s.s.</i>, <i>An. koliensis</i>, <i>An. farauti s.s.</i>, and <i>An. farauti</i> 4. I interpret these results in the context of vector control and disease transmission in Papua New Guinea.</p><p>I sequenced the mitochondrial and nuclear genomes of these mosquitoes and used phylogenetic analysis to estimate species divergence. Phylogenetic analysis indicates that AP species rapidly diverged in the past and current species are 6-9% divergent. I confirmed that species have evolved independently by searching the nuclear genome for signs of introgression. Analyses of more than 50Mb of orthologous nuclear DNA sequences revealed no evidence of contemporary introgression among AP species studied.</p><p>Currently, the host feeding patterns of AP species are not well defined. I developed a novel targeted high-throughput sequencing technique that provides an unbiased and comprehensive perspective on the composition of each mosquito blood meal. I tested this method on 442 female AP mosquitoes from Papua New Guinea. My analyses revealed that 16.3% of the mosquitoes fed on more than one host and predominately fed on humans, dogs and pigs, but also fed on a marsupial species and bats.</p> <p>Overall, my findings reveal the utility of high-throughput sequencing for exploring the biology of understudied species. The data and techniques generated in my dissertation will aid future studies of understudied mosquitoes and enable us to better understand vector related traits. My method to query blood meals can then be used in addition to genomic data to understand mosquito behavior but could also be used to study the feeding patterns of other insect vectors of diseases.</p> 2016-06-01 English text Case Western Reserve University School of Graduate Studies / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=case1455900373 http://rave.ohiolink.edu/etdc/view?acc_num=case1455900373 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Biology Genetics |
| spellingShingle |
Biology Genetics Logue, Kyle J. Genomic Analyses of the <i>Anopheles punctulatus</i> Group: Insights into Mosquito Biology and Implications for Vector Control and Disease Transmission |
| author |
Logue, Kyle J. |
| author_facet |
Logue, Kyle J. |
| author_sort |
Logue, Kyle J. |
| title |
Genomic Analyses of the <i>Anopheles punctulatus</i> Group: Insights into Mosquito Biology and Implications for Vector Control and Disease Transmission |
| title_short |
Genomic Analyses of the <i>Anopheles punctulatus</i> Group: Insights into Mosquito Biology and Implications for Vector Control and Disease Transmission |
| title_full |
Genomic Analyses of the <i>Anopheles punctulatus</i> Group: Insights into Mosquito Biology and Implications for Vector Control and Disease Transmission |
| title_fullStr |
Genomic Analyses of the <i>Anopheles punctulatus</i> Group: Insights into Mosquito Biology and Implications for Vector Control and Disease Transmission |
| title_full_unstemmed |
Genomic Analyses of the <i>Anopheles punctulatus</i> Group: Insights into Mosquito Biology and Implications for Vector Control and Disease Transmission |
| title_sort |
genomic analyses of the <i>anopheles punctulatus</i> group: insights into mosquito biology and implications for vector control and disease transmission |
| publisher |
Case Western Reserve University School of Graduate Studies / OhioLINK |
| publishDate |
2016 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=case1455900373 |
| work_keys_str_mv |
AT loguekylej genomicanalysesoftheianophelespunctulatusigroupinsightsintomosquitobiologyandimplicationsforvectorcontrolanddiseasetransmission |
| _version_ |
1719439508287520768 |