Identification and characterization of actin-regulatory proteins in the hair cell's cuticular plate
| Main Author: | |
|---|---|
| Language: | English |
| Published: |
Case Western Reserve University School of Graduate Studies / OhioLINK
2016
|
| Subjects: | |
| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=case1448649914 |
| id |
ndltd-OhioLink-oai-etd.ohiolink.edu-case1448649914 |
|---|---|
| record_format |
oai_dc |
| spelling |
ndltd-OhioLink-oai-etd.ohiolink.edu-case14486499142021-08-03T06:34:09Z Identification and characterization of actin-regulatory proteins in the hair cell's cuticular plate Pollock, Lana Mary Biomedical Research Genetics The mechanosensitive hair cells of the inner ear are crucial for hearing and vestibular function. Each hair cell detects mechanical stimuli associated with sound or head movement with the hair bundle, consisting of an array of actin-based stereocilia. Each stereocilium inserts as a rootlet into a dense filamentous actin mesh known as the cuticular plate. Disruption of the actin bundles forming the stereocilia results in hearing and balance defects. The cuticular plate is thought to hold the stereocilia in place. However, the precise role of the cuticular plate in hair bundle development, maintenance, and hearing remains unknown. Ultrastructural studies have revealed a complex cytoskeletal architecture, but a lack of knowledge of proteins inhabiting the cuticular plate have hindered our understanding of its functions. Additionally, if and how the cuticular plate is physically connected to the underlying region of the hair cell’s body, which contains columns of microtubules has not been previously established. Here we identify two previously unknown actin-binding proteins localized to the hair cell’s cuticular plate, which may play important roles in shaping this unique and mysterious structure.One of these proteins is actin crosslinking family 7 (ACF7), a specktraplakin protein that binds both actin and microtubules. ACF7 localizes to the cuticular plate and extends into the region of the underlying somatic microtubules. ACF7 is the first actin and microtubule cross-linking protein to be localized specifically in the region of the cuticular plate and may physically link cuticular plate actin to somatic microtubules.Supervillin (SVIL) is the first actin-cross-linking protein to be found localized exclusively to the cuticular plate, where it may hold together the actin mesh. In the mouse cochlea, SVIL also localizes to the apicolateral edges of non-sensory supporting cells, adjacent to the junctions connecting them with surrounding hair cells. SVIL may be involved in bundling and stabilizing actin filaments associated with these junctions. SVIL likely plays a role in maintaining integrity of the cochlear reticular lamina under sound-induced mechanical stresses. Further characterization of the roles of ACF7 and SVIL in hair cells may help us elucidate the structure and function of the poorly understood cuticular plate. 2016-01-27 English text Case Western Reserve University School of Graduate Studies / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=case1448649914 http://rave.ohiolink.edu/etdc/view?acc_num=case1448649914 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
| collection |
NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Biomedical Research Genetics |
| spellingShingle |
Biomedical Research Genetics Pollock, Lana Mary Identification and characterization of actin-regulatory proteins in the hair cell's cuticular plate |
| author |
Pollock, Lana Mary |
| author_facet |
Pollock, Lana Mary |
| author_sort |
Pollock, Lana Mary |
| title |
Identification and characterization of actin-regulatory proteins in the hair cell's cuticular plate |
| title_short |
Identification and characterization of actin-regulatory proteins in the hair cell's cuticular plate |
| title_full |
Identification and characterization of actin-regulatory proteins in the hair cell's cuticular plate |
| title_fullStr |
Identification and characterization of actin-regulatory proteins in the hair cell's cuticular plate |
| title_full_unstemmed |
Identification and characterization of actin-regulatory proteins in the hair cell's cuticular plate |
| title_sort |
identification and characterization of actin-regulatory proteins in the hair cell's cuticular plate |
| publisher |
Case Western Reserve University School of Graduate Studies / OhioLINK |
| publishDate |
2016 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=case1448649914 |
| work_keys_str_mv |
AT pollocklanamary identificationandcharacterizationofactinregulatoryproteinsinthehaircellscuticularplate |
| _version_ |
1719439235877961728 |