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ndltd-OhioLink-oai-etd.ohiolink.edu-case13213682492021-08-03T05:34:10Z Trehalose-Based Biofuel Cells Rasmussen, Michelle <p>A trehalose biosensor has been developed which incorporates two enzymes, trehalase (Tre) and glucose oxidase (GOx). The enzymes are covalently bound to the surface of Pt through a functionalized thiol linker. The catalytic properties of the sensor were examined by chemical and electrochemical methods in aqueous phosphate buffer solutions (PBS, pH 7.4). The trehalose detection is accomplished by measuring the steady state current at +0.6 V vs. Ag/AgCl due to the oxidation of hydrogen peroxide generated by the GOx-mediated oxidation of glucose (Gl) produced by the Tre-mediated dissociation of Tr. Plots of the steady state currents versus Tr concentration over the range examined, i.e. 5 to 25 mM, were found to be linear.</p><p> A trehalose/oxygen biofuel cell has been designed and constructed. The trehalose anode consists of a carbon electrode coated with a film of two enzymes, trehalase and glucose oxidase, bound to an osmium redox polymer. The oxygen cathode is a carbon electrode coated with a second osmium polymer and a third enzyme, bilirubin oxidase,. The power of the biofuel cell was measured in three environments. In 50 mM trehalose solution in PBS, the maximum power output is 15 µW/cm<sup>2</sup> at 0.25 V. In a shiitake mushroom, the power generated is 1.2 µW/cm<sup>2</sup> at 0.2 V; however, the power output is not stable and decreases to zero after only one hour. When the two electrodes are implanted in a cockroach with the hemolymph acting as electrolyte, the power output is 57 µW/cm<sup>2</sup> at 0.2 V. This result is quite stable and after 2.5 hours decreases by approximately 5%.</p> 2012-01-31 English text Case Western Reserve University School of Graduate Studies / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=case1321368249 http://rave.ohiolink.edu/etdc/view?acc_num=case1321368249 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
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NDLTD
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| language |
English
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NDLTD
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| author |
Rasmussen, Michelle
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| spellingShingle |
Rasmussen, Michelle
Trehalose-Based Biofuel Cells
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| author_facet |
Rasmussen, Michelle
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| author_sort |
Rasmussen, Michelle
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| title |
Trehalose-Based Biofuel Cells
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| title_short |
Trehalose-Based Biofuel Cells
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| title_full |
Trehalose-Based Biofuel Cells
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| title_fullStr |
Trehalose-Based Biofuel Cells
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| title_full_unstemmed |
Trehalose-Based Biofuel Cells
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| title_sort |
trehalose-based biofuel cells
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| publisher |
Case Western Reserve University School of Graduate Studies / OhioLINK
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| publishDate |
2012
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http://rave.ohiolink.edu/etdc/view?acc_num=case1321368249
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AT rasmussenmichelle trehalosebasedbiofuelcells
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1719421889891270656
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