Optode-bead-based Functional Chemical Imaging of 2D Substrates
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Case Western Reserve University School of Graduate Studies / OhioLINK
2011
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=case1307727754 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-case13077277542021-08-03T05:34:10Z Optode-bead-based Functional Chemical Imaging of 2D Substrates Ahuja, Punkaj N. Biomedical Engineering Imaging electrochemical electrochemistry scanning electrochemical microscopy secm ion pH proton multicellular tumor spheroids MCTS bulk optode We introduce a new technology for functional imaging of surfaces using microscopic optode beads. The imaging technology produces a map of 2D chemical distribution, obtained continuously and simultaneously at each pixel using a charged coupled device (CCD) camera. Ionic distributions can be imaged with this technique using ionophore-chromoionophores interactions in microscopic optode beads or membranes. Response to pH diffusion from a 140 um channel were measured. Lateral and spherical diffusion processes are compared. Data was resolved at each pixel using linear interpolation of three closest beads with Delaunay triangulation and color- concentration maps were created assuming circular symmetry. The approach is similar to Scanning Electrochemical Microscopy, an imaging modality that relies on the scanning of an electrochemical microsensor along a surface of interest. Potential or current measures local concentrations of an analyte at each scanned position. Scanning of the microelectrode tip is, however, time consuming and the tip itself may run into physical obstruction at uneven surfaces. Functional optode-bead imaging provides an imaging modality that can produce similar functional 2D maps as scanning electrochemical microscopy does, but without the need for physical scanning of an electrode. The functional optode bead technology has a variety of potential including measurements in systems scanning electrochemical microscopy could not be applied. Some biomedical applications include imaging of pH in epithelial cell monolayers and multicellular tumor spheroids (MCTS), which are meant to represent tumor microenvironments. 2011-06-30 English text Case Western Reserve University School of Graduate Studies / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=case1307727754 http://rave.ohiolink.edu/etdc/view?acc_num=case1307727754 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Biomedical Engineering Imaging electrochemical electrochemistry scanning electrochemical microscopy secm ion pH proton multicellular tumor spheroids MCTS bulk optode |
| spellingShingle |
Biomedical Engineering Imaging electrochemical electrochemistry scanning electrochemical microscopy secm ion pH proton multicellular tumor spheroids MCTS bulk optode Ahuja, Punkaj N. Optode-bead-based Functional Chemical Imaging of 2D Substrates |
| author |
Ahuja, Punkaj N. |
| author_facet |
Ahuja, Punkaj N. |
| author_sort |
Ahuja, Punkaj N. |
| title |
Optode-bead-based Functional Chemical Imaging of 2D Substrates |
| title_short |
Optode-bead-based Functional Chemical Imaging of 2D Substrates |
| title_full |
Optode-bead-based Functional Chemical Imaging of 2D Substrates |
| title_fullStr |
Optode-bead-based Functional Chemical Imaging of 2D Substrates |
| title_full_unstemmed |
Optode-bead-based Functional Chemical Imaging of 2D Substrates |
| title_sort |
optode-bead-based functional chemical imaging of 2d substrates |
| publisher |
Case Western Reserve University School of Graduate Studies / OhioLINK |
| publishDate |
2011 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=case1307727754 |
| work_keys_str_mv |
AT ahujapunkajn optodebeadbasedfunctionalchemicalimagingof2dsubstrates |
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1719421855988711424 |