Complex N-glycans are Required for Carbohydrate Antigen Presentation by MHC II

Complex N-glycans are Required for Carbohydrate Antigen Presentation by MHC II

Bibliographic Details
Main Author: Zhao, Fan
Language:English
Published: Case Western Reserve University School of Graduate Studies / OhioLINK 2010
Subjects:
Immunology
MHC II
Glycosylation
Carbohydrate Antigen Presentation
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=case1278621860
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-case12786218602021-08-03T05:33:51Z Complex N-glycans are Required for Carbohydrate Antigen Presentation by MHC II Zhao, Fan Immunology MHC II Glycosylation Carbohydrate Antigen Presentation Glycosylation of immune proteins plays critical roles in the adaptive immune system. Complexasparagine (N)-linked glycans are involved in directing the proper protein folding andtrafficking to the cell surface, protection from the proteolytic cleavage, and promoting theappropriate cell-cell adhesion and communication. Our study shows that elimination ofcomplex N-linked glycans on MHC II reduced the presentation of a novel zwitterionicpolysaccharide antigen PSA which expressed by B. fragilis but not conventional peptides inlive antigen presenting cells (APCs). The binding assay demonstrated that full size complexN-glycans are necessary for PSA but not conventional peptide antigen binding to MHC II. Tcell activation assay conducted in mouse bone marrow-derived dendritic cells (BMDCs) showsthe in vitro evidence that PSA-mediated T cell activation is significantly reduced due to theblockade of complex N-glycans of MHC II in APCs. However, lacking N-glycans on MHC IIdoes not affect OT-II T cell activation with OVA peptide. These results reveal thatN-glycosylation of MHC II directly influences PSA but not conventional protein antigenpresentation and downstream T cell activation. 2010 English text Case Western Reserve University School of Graduate Studies / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=case1278621860 http://rave.ohiolink.edu/etdc/view?acc_num=case1278621860 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Immunology
MHC II
Glycosylation
Carbohydrate Antigen Presentation
spellingShingle Immunology
MHC II
Glycosylation
Carbohydrate Antigen Presentation
Zhao, Fan
Complex N-glycans are Required for Carbohydrate Antigen Presentation by MHC II
author Zhao, Fan
author_facet Zhao, Fan
author_sort Zhao, Fan
title Complex N-glycans are Required for Carbohydrate Antigen Presentation by MHC II
title_short Complex N-glycans are Required for Carbohydrate Antigen Presentation by MHC II
title_full Complex N-glycans are Required for Carbohydrate Antigen Presentation by MHC II
title_fullStr Complex N-glycans are Required for Carbohydrate Antigen Presentation by MHC II
title_full_unstemmed Complex N-glycans are Required for Carbohydrate Antigen Presentation by MHC II
title_sort complex n-glycans are required for carbohydrate antigen presentation by mhc ii
publisher Case Western Reserve University School of Graduate Studies / OhioLINK
publishDate 2010
url http://rave.ohiolink.edu/etdc/view?acc_num=case1278621860
work_keys_str_mv AT zhaofan complexnglycansarerequiredforcarbohydrateantigenpresentationbymhcii
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