Exploring the Three-Dimensional Regional Myocardial Function in Transgenic Mouse Models of Cardiac Diseases using Novel MR Tissue Tracking Techniques
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Case Western Reserve University School of Graduate Studies / OhioLINK
2009
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=case1247260314 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-case12472603142021-08-03T05:33:16Z Exploring the Three-Dimensional Regional Myocardial Function in Transgenic Mouse Models of Cardiac Diseases using Novel MR Tissue Tracking Techniques Zhong, Jia Biomedical Research Engineering transgenic mouse models cardiac diseases spatial modulation of magnetization (SPAMM) tagging harmonic phase (HARP) displacement encoding with stimulated echoes (DENSE) strain regional myocardial function <p>The advent of the genomic age is revolutionizing the experimental cardiovascular research irreversibly. Dissecting molecular switches that control the changes of cardiac physiology with transgenic mouse models has proven to offer important insights into the control of cardiac function. The integration of novel MR tissue tracking techqniques with these genetically manipulated mice may allow comprehensive characterization of contractile dysfunction non-invasively at the earliest diseased stages, thus facilitate our understanding of the pathogenesis of human cardiac diseases. </p><p>In the current thesis, we aimed at developing fast and accurate MR tissue tracking techniques and applying them for the assessment of ventricular function in transgenic mouse models of cardiac diseases. First, spatial modulation of magnetization (SPAMM) tagging was implemented in the mouse heart; and a 3D SPAMM tagging analysis method was developed based on harmonic phase (HARP) and homogeneous strain analysis. Using this 3D tagging analysis method, longitudinal strain and circumferential-longitudinal shear was quantified in addition to the 2D ventricular wall strain. Second, to improve the limited tagging resolution of existing SPAMM techniques, a HARP-based high-resolution tagging analysis method was proposed in mouse. The utility of such method was demostrated by quantifying the transmural heterogeneity of the left ventricle. Third, a 2D multi-phase displacement encoding with stimulated echoes (DENSE) imaging and analysis method was developed which allows direct and automatic Lagrangian strain quantification with high spatial and temporal resolution. Additionally, the utility of this multi-phase DENSE method was demonstrated in mouse both at baseline and with high workload. Functional enhancement was identified upon dobutamine stimulation both at the global and the regional levels. Fourth, for the evaluation of longitudinal wall motion within the short axis (SA) plane, 2D multi-phase DENSE imaging method was extended to the third dimension. Last, the application of MR tagging and DENSE methods was investigated in diabetic mouse hearts with cardiac-specific GLUT1-overexpression. Normalized cardiac function was found in diabetic mice with enhanced glucose metabolism through GLUT1-overexpression. Our results demonstrated the capability of MR tagging and DENSE in delineating left ventricular function in the mouse heart.</p> 2009 English text Case Western Reserve University School of Graduate Studies / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=case1247260314 http://rave.ohiolink.edu/etdc/view?acc_num=case1247260314 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Biomedical Research Engineering transgenic mouse models cardiac diseases spatial modulation of magnetization (SPAMM) tagging harmonic phase (HARP) displacement encoding with stimulated echoes (DENSE) strain regional myocardial function |
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Biomedical Research Engineering transgenic mouse models cardiac diseases spatial modulation of magnetization (SPAMM) tagging harmonic phase (HARP) displacement encoding with stimulated echoes (DENSE) strain regional myocardial function Zhong, Jia Exploring the Three-Dimensional Regional Myocardial Function in Transgenic Mouse Models of Cardiac Diseases using Novel MR Tissue Tracking Techniques |
| author |
Zhong, Jia |
| author_facet |
Zhong, Jia |
| author_sort |
Zhong, Jia |
| title |
Exploring the Three-Dimensional Regional Myocardial Function in Transgenic Mouse Models of Cardiac Diseases using Novel MR Tissue Tracking Techniques |
| title_short |
Exploring the Three-Dimensional Regional Myocardial Function in Transgenic Mouse Models of Cardiac Diseases using Novel MR Tissue Tracking Techniques |
| title_full |
Exploring the Three-Dimensional Regional Myocardial Function in Transgenic Mouse Models of Cardiac Diseases using Novel MR Tissue Tracking Techniques |
| title_fullStr |
Exploring the Three-Dimensional Regional Myocardial Function in Transgenic Mouse Models of Cardiac Diseases using Novel MR Tissue Tracking Techniques |
| title_full_unstemmed |
Exploring the Three-Dimensional Regional Myocardial Function in Transgenic Mouse Models of Cardiac Diseases using Novel MR Tissue Tracking Techniques |
| title_sort |
exploring the three-dimensional regional myocardial function in transgenic mouse models of cardiac diseases using novel mr tissue tracking techniques |
| publisher |
Case Western Reserve University School of Graduate Studies / OhioLINK |
| publishDate |
2009 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=case1247260314 |
| work_keys_str_mv |
AT zhongjia exploringthethreedimensionalregionalmyocardialfunctionintransgenicmousemodelsofcardiacdiseasesusingnovelmrtissuetrackingtechniques |
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1719421656440504320 |