Functional and molecular characterization of a candidate gene family for thet-complex responder locus
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Case Western Reserve University School of Graduate Studies / OhioLINK
1992
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=case1055961951 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-case10559619512021-08-03T05:30:45Z Functional and molecular characterization of a candidate gene family for thet-complex responder locus Bullard, Daniel Charles candidate gene family thet complex responder locus Male transmission ratio distortion (TRD) is a property of mouse t haplotypes which requires the t complex responder locus (Tcr). Tcr has been localized to a 70-160kb region in t haplotypes. In previous work, a family of genes (the Tcp10 genes) was identified within this region that encodes testes-specific transcripts. One member of this gene family, called Tcp10b t, shows several properties which make it a strong a candidate for the responder. Tcp10b t maps to the same genetic subregion (T66B) as the responder, is expressed exclusively in the testes, and is the only detectable testes-expressed gene that has been identified so far in the T66B locus. In order to determine any possible relationships between the Tcp10 genes and the responder, a molecular and functional analysis of this gene family was initiated. The four members of the Tcp10 gene family were cloned and mapped to specific genetic subregions in the responder locus, and each gene was mapped for several different restriction enzymes. The results of these studies indicate that the Tcp10 genes are highly similar to one another and were created by recent, complex duplication events. In addition, the exon/intron structure of a representative Tcp10 gene was determined. The Tcp10 genes contain 12 exons whi ch span approximately 21kb of DNA. In order to analyze the Tcp10b t gene for responder activity, a cosmid clone containing this gene was used to make transgenic mice. Transgenic males were subsequently bred into several different genetic backgrounds (including some which contained partial or complete t haplotypes) and these mice were assayed for responder activity associated with the Tcp10b t transgene. The breeding studies, however, did not show any strong evidence that the Tcp10b t gene has responder activity. 1992 English text Case Western Reserve University School of Graduate Studies / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=case1055961951 http://rave.ohiolink.edu/etdc/view?acc_num=case1055961951 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
| collection |
NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
candidate gene family thet complex responder locus |
| spellingShingle |
candidate gene family thet complex responder locus Bullard, Daniel Charles Functional and molecular characterization of a candidate gene family for thet-complex responder locus |
| author |
Bullard, Daniel Charles |
| author_facet |
Bullard, Daniel Charles |
| author_sort |
Bullard, Daniel Charles |
| title |
Functional and molecular characterization of a candidate gene family for thet-complex responder locus |
| title_short |
Functional and molecular characterization of a candidate gene family for thet-complex responder locus |
| title_full |
Functional and molecular characterization of a candidate gene family for thet-complex responder locus |
| title_fullStr |
Functional and molecular characterization of a candidate gene family for thet-complex responder locus |
| title_full_unstemmed |
Functional and molecular characterization of a candidate gene family for thet-complex responder locus |
| title_sort |
functional and molecular characterization of a candidate gene family for thet-complex responder locus |
| publisher |
Case Western Reserve University School of Graduate Studies / OhioLINK |
| publishDate |
1992 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=case1055961951 |
| work_keys_str_mv |
AT bullarddanielcharles functionalandmolecularcharacterizationofacandidategenefamilyforthetcomplexresponderlocus |
| _version_ |
1719421089328660480 |