Diversity of β-Lactamase Genes in Gram-Negative Soil Bacteria from Northwest Ohio
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| Language: | English |
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Bowling Green State University / OhioLINK
2019
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1566553116919146 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-bgsu15665531169191462021-08-03T07:12:54Z Diversity of β-Lactamase Genes in Gram-Negative Soil Bacteria from Northwest Ohio Albaaj, Mohammed Microbiology Soil Sciences Biology gram-negative soil bacteria beta-lactamase antibiotic resistance CTX-M Rahnella Pseudomonas lateral gene transfer horizontal gene transfer The development of resistance to numerous antibiotics by bacterial pathogens presents a matter of growing concern in the healthcare community. The inability of standard antibiotics to resolve once easily treated infections has put modern populations at risk and has resulted in a significantly increased economic burden to health care systems worldwide. It is critical to develop new drugs and strategies to combat this growing healthcare challenge. Effective drug design and strategy requires understanding of resistance at the molecular level. This study considers the resistance to one major class of antibiotics, the β-lactams, from the perspective of environmental organisms that carry genes that encode enzymes that inactive these antibiotics. These microbial populations serve as a potential reservoir in which resistance mechanisms evolve and from which they can potentially move into clinically relevant settings. I recovered bacteria from environmental soil-based sources using a selective medium (MacConkey agar) supplemented with ampicillin to isolate bacteria with resistance to this mainstay β-lactam antibiotic. Isolates were screened for the presence of genes encoding β-lactamase proteins in of the CTX-M type. This highly prevalent group of enzymes confer resistance to bacteria by mediating the hydrolysis and inactivation of a wide variety of β-lactam antibiotics. Following identification, the sequences of CTX-M-type β-lactamase genes were determined, with the predicted amino acid sequences of their encoded proteins compared to each other and to previously identified CTX-M β-lactamases. The β-lactamases identified shared high degrees of sequence identity indicative of a close evolutionary relationship, yet occurred in bacterial isolates that appeared to be evolutionarily more distant based on divergent 16S rRNA gene sequences. These observations suggested the possibility that the CTX-M-type β-lactamase genes examined were dispersed by lateral gene transfer, consistent with other studies. 2019-11-26 English text Bowling Green State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1566553116919146 http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1566553116919146 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Microbiology Soil Sciences Biology gram-negative soil bacteria beta-lactamase antibiotic resistance CTX-M Rahnella Pseudomonas lateral gene transfer horizontal gene transfer |
| spellingShingle |
Microbiology Soil Sciences Biology gram-negative soil bacteria beta-lactamase antibiotic resistance CTX-M Rahnella Pseudomonas lateral gene transfer horizontal gene transfer Albaaj, Mohammed Diversity of β-Lactamase Genes in Gram-Negative Soil Bacteria from Northwest Ohio |
| author |
Albaaj, Mohammed |
| author_facet |
Albaaj, Mohammed |
| author_sort |
Albaaj, Mohammed |
| title |
Diversity of β-Lactamase Genes in Gram-Negative Soil Bacteria from Northwest Ohio |
| title_short |
Diversity of β-Lactamase Genes in Gram-Negative Soil Bacteria from Northwest Ohio |
| title_full |
Diversity of β-Lactamase Genes in Gram-Negative Soil Bacteria from Northwest Ohio |
| title_fullStr |
Diversity of β-Lactamase Genes in Gram-Negative Soil Bacteria from Northwest Ohio |
| title_full_unstemmed |
Diversity of β-Lactamase Genes in Gram-Negative Soil Bacteria from Northwest Ohio |
| title_sort |
diversity of β-lactamase genes in gram-negative soil bacteria from northwest ohio |
| publisher |
Bowling Green State University / OhioLINK |
| publishDate |
2019 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1566553116919146 |
| work_keys_str_mv |
AT albaajmohammed diversityofblactamasegenesingramnegativesoilbacteriafromnorthwestohio |
| _version_ |
1719456178166038528 |