A Cytochemical and Physiological Study of Photosynthetic and Respiratory Electron Reactions in Phormidium Luridum Var. Olivacea
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| Language: | English |
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Bowling Green State University / OhioLINK
1976
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1566463048506066 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-bgsu15664630485060662021-08-03T07:12:44Z A Cytochemical and Physiological Study of Photosynthetic and Respiratory Electron Reactions in Phormidium Luridum Var. Olivacea Sun, Donald C. Biology Various cyanobacterial inhibitors and a cyanobacteriocidal toxin produced by Bdellovibrio bacteriovorus (BdAS) were compared for their effect on Phormidium luridum, with regard to morphology, ultrastructure, photosynthesis, respiration, in vitro ferricyanide reduction and electron transport cytochemistry. Electron microscopy reveals a similar cellular response to different cyanobacterial inhibitors, i.e., dichloro-methylurea (DCMU); potassium cyanide (KCN); and BdAS, i.e., formation of intrathylakoidal spaces. At 2000 fc, whole cells of P. luridum produced 405.4 ul 02/mg chl a/hr while fresh spheroplasts produced 326.7 ul 02mg chl a/hr, an efficiency of 80%. The respiration rate of spheroplasts was higher than whole cells.The rate of ferricyanide reduction by whole cells of P. luridum was 126.6 uM/mg chl a/hr. Higher ferricyanide reduction rates were observed in polymyxin B-treated cells (451.2 uM/mg chl a/hr) and spheroplasts (331.2 uM/mg chl a/hr). The ferricyanide reduction rate was stimulated by 0-phenanthroline and BdAS and decreased by DCMU, KCN, ouabain and rotenone.Copper ferrocyanide electron cytochemistry was non-specific except in cells treated with sodium lauryl sulfate. Deposition of 3, 3-diaminobenzidine (DAB) was seen in the intrathylakoidal space. Cytochemical localization, using tetranitroblue tetrazolium (TNBT) was slightly inhibited by BdAS and severely inhibited by ouabain and polymyxin B. Cytochemical results showed that the photosynthetic lamellae of P. luridum also functioned as reactive sites for respiration. The intrathylakoidal space of P. luridum possessed cytochrome c oxidase activity similar to that localized in intracristal spaces of eukaryotic cells. Physiological and cytochemical data indicated that the inhibitory mechanism of BdAS in P. luridum was not identical to other inhibitors used in this study. 1976 English text Bowling Green State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1566463048506066 http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1566463048506066 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Biology |
| spellingShingle |
Biology Sun, Donald C. A Cytochemical and Physiological Study of Photosynthetic and Respiratory Electron Reactions in Phormidium Luridum Var. Olivacea |
| author |
Sun, Donald C. |
| author_facet |
Sun, Donald C. |
| author_sort |
Sun, Donald C. |
| title |
A Cytochemical and Physiological Study of Photosynthetic and Respiratory Electron Reactions in Phormidium Luridum Var. Olivacea |
| title_short |
A Cytochemical and Physiological Study of Photosynthetic and Respiratory Electron Reactions in Phormidium Luridum Var. Olivacea |
| title_full |
A Cytochemical and Physiological Study of Photosynthetic and Respiratory Electron Reactions in Phormidium Luridum Var. Olivacea |
| title_fullStr |
A Cytochemical and Physiological Study of Photosynthetic and Respiratory Electron Reactions in Phormidium Luridum Var. Olivacea |
| title_full_unstemmed |
A Cytochemical and Physiological Study of Photosynthetic and Respiratory Electron Reactions in Phormidium Luridum Var. Olivacea |
| title_sort |
cytochemical and physiological study of photosynthetic and respiratory electron reactions in phormidium luridum var. olivacea |
| publisher |
Bowling Green State University / OhioLINK |
| publishDate |
1976 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1566463048506066 |
| work_keys_str_mv |
AT sundonaldc acytochemicalandphysiologicalstudyofphotosyntheticandrespiratoryelectronreactionsinphormidiumluridumvarolivacea AT sundonaldc cytochemicalandphysiologicalstudyofphotosyntheticandrespiratoryelectronreactionsinphormidiumluridumvarolivacea |
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