Polyol Pathway Enzyme Activities in the Mouse Placenta and the Uterus During Pregnancy and Pseudopregnancy
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Bowling Green State University / OhioLINK
1974
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1566297702063815 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-bgsu15662977020638152021-08-03T07:12:35Z Polyol Pathway Enzyme Activities in the Mouse Placenta and the Uterus During Pregnancy and Pseudopregnancy Parrish, John Wesley, Jr. Biology The purpose of this study was to investigate the enzyme activities of the polyol pathway in the placenta and uterus during pregnancy and pseudopregnancy. Mice were sacrificed at 14, 16, 18, 19 and 20 days of gestation (vaginal plug= day 1). Placentae and juxtaplacental uteri were assayed, spectrophotometrically, for aldose reductase (AR) and sorbitol dehydrogenase (SDH). Placental AR levels followed a significantly linear decrease in enzyme activity between days 14 and 20 (day of parturition). A similar decrease in AR activity was found to occur in the juxtaplacental uterus. Both placental and uterine AR activities were significantly greater during the early part of the last week than near term. SDH enzyme activities, in contrast, were found to remain relatively constant throughout gestation in both the placenta and the uterus. Neither AR nor SDH were detectable in the uterus of mice ovariectomized for two weeks. Exogenous estrogen was found to induce both enzymes to near normal levels. Induction of deciduoma in the uterus resulted in a significant increase in AR in pseudopregnant mice. SDH was not significantly altered by deciduoma induction. Enzyme kinetic data indicated an apparent Michaelis-Menten constant (Km) of AR for D-glucose of about 24mM, while the Km of SDH for sorbitol was found to be about lOmM. The Km of SDH for NAO+ as coenzyme was determined to be about 0.22mM. It was concluded that the changes in polyol pathway enzyme activities might correlate with the decrease in pentose-phosphate shunt (PPS) activity which has been recorded in other mammals, and that the polyol pathway may facilitate enhanced PPS activity during gestation. It was also suggested that the determined high Km of AR would result in increased cellular NADP+ levels which could facilitate increased NADP+-dependent PPS activity during rapid, transient increases in blood glucose levels. The increased placental AR activity at term was suggested to be a possible ancillary osmotic mechanism involved in separation of the placenta and uterus at parturition. 1974 English text Bowling Green State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1566297702063815 http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1566297702063815 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Biology |
| spellingShingle |
Biology Parrish, John Wesley, Jr. Polyol Pathway Enzyme Activities in the Mouse Placenta and the Uterus During Pregnancy and Pseudopregnancy |
| author |
Parrish, John Wesley, Jr. |
| author_facet |
Parrish, John Wesley, Jr. |
| author_sort |
Parrish, John Wesley, Jr. |
| title |
Polyol Pathway Enzyme Activities in the Mouse Placenta and the Uterus During Pregnancy and Pseudopregnancy |
| title_short |
Polyol Pathway Enzyme Activities in the Mouse Placenta and the Uterus During Pregnancy and Pseudopregnancy |
| title_full |
Polyol Pathway Enzyme Activities in the Mouse Placenta and the Uterus During Pregnancy and Pseudopregnancy |
| title_fullStr |
Polyol Pathway Enzyme Activities in the Mouse Placenta and the Uterus During Pregnancy and Pseudopregnancy |
| title_full_unstemmed |
Polyol Pathway Enzyme Activities in the Mouse Placenta and the Uterus During Pregnancy and Pseudopregnancy |
| title_sort |
polyol pathway enzyme activities in the mouse placenta and the uterus during pregnancy and pseudopregnancy |
| publisher |
Bowling Green State University / OhioLINK |
| publishDate |
1974 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1566297702063815 |
| work_keys_str_mv |
AT parrishjohnwesleyjr polyolpathwayenzymeactivitiesinthemouseplacentaandtheuterusduringpregnancyandpseudopregnancy |
| _version_ |
1719456041361473536 |