Identifying biosynthetic gene clusters whose products inhibit cystic fibrosis derived pathogens
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Bowling Green State University / OhioLINK
2018
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1530795688583696 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-bgsu15307956885836962021-08-03T07:07:21Z Identifying biosynthetic gene clusters whose products inhibit cystic fibrosis derived pathogens Basalla, Joseph Biology Microbiology Molecular Biology Antibiotic resistance cystic fibrosis environmental microbiology Pseudomonas The emergence of pathogens resistant to all known antibiotics has become a serious threatto our healthcare systems. In 2050, infections from antibiotic resistant bacteria are predicted tobecome the leading cause of death worldwide, reaching over 300,000 per year in the U.S. alone.Unfortunately, pharmaceutical companies have stopped research and development of new drugsdue to low profitability, so university research labs have begun leading efforts to discover newcompounds. In concordance with this, the Wildschutte lab uses environmental Pseudomonas spp.as a source for novel antibiotics. We hypothesize that pathogenic bacteria have evolvedadaptations to their host, and that these adaptations may result in a decreased fitness whencompeted against bacteria from non-host, natural environments. To support this, we have shownthat environmental Pseudomonas from different habitats produce diverse compounds that inhibitpathogens isolated from human hosts. Specifically, 495 environmental Pseudomonas strains wereisolated from U.S. derived soil, freshwater lakes in Hungary, and estuaries in Germany. Theseenvironmental strains were then competed against a panel of 70 pathogens isolated from thelungs of cystic fibrosis patients. In total, over 2,000 of these interactions were seen as inhibitory,suggesting that the environmental strains represent an underutilized source of antibiotics.Additionally, environmental strains that were shown to inhibit a number of pathogens werestudied using transposon-mutagenesis to uncover the underlying genetic elements responsible forinhibition. Through this process, we have identified a number of putative metabolites capable ofinhibiting the growth of human pathogens. 2018-07-23 English text Bowling Green State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1530795688583696 http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1530795688583696 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Biology Microbiology Molecular Biology Antibiotic resistance cystic fibrosis environmental microbiology Pseudomonas |
| spellingShingle |
Biology Microbiology Molecular Biology Antibiotic resistance cystic fibrosis environmental microbiology Pseudomonas Basalla, Joseph Identifying biosynthetic gene clusters whose products inhibit cystic fibrosis derived pathogens |
| author |
Basalla, Joseph |
| author_facet |
Basalla, Joseph |
| author_sort |
Basalla, Joseph |
| title |
Identifying biosynthetic gene clusters whose products inhibit cystic fibrosis derived pathogens |
| title_short |
Identifying biosynthetic gene clusters whose products inhibit cystic fibrosis derived pathogens |
| title_full |
Identifying biosynthetic gene clusters whose products inhibit cystic fibrosis derived pathogens |
| title_fullStr |
Identifying biosynthetic gene clusters whose products inhibit cystic fibrosis derived pathogens |
| title_full_unstemmed |
Identifying biosynthetic gene clusters whose products inhibit cystic fibrosis derived pathogens |
| title_sort |
identifying biosynthetic gene clusters whose products inhibit cystic fibrosis derived pathogens |
| publisher |
Bowling Green State University / OhioLINK |
| publishDate |
2018 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1530795688583696 |
| work_keys_str_mv |
AT basallajoseph identifyingbiosyntheticgeneclusterswhoseproductsinhibitcysticfibrosisderivedpathogens |
| _version_ |
1719453876243922944 |