Functional Analysis and Characterization of Transporter of Putrescine and Spermidine (TOPAS1) in Phytophthora parasitica
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Bowling Green State University / OhioLINK
2017
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=bgsu149874256456224 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-bgsu1498742564562242021-08-03T07:02:55Z Functional Analysis and Characterization of Transporter of Putrescine and Spermidine (TOPAS1) in Phytophthora parasitica Chakrabarti, Nilanjana Molecular Biology polyamines conserved family phytophthora parasitica transporters Sequence data and gene models are now available for 19 oomycete genomes. Only a very small number of oomycete genes have been functionally characterized, because oomycetes are in the Kingdom Stramenopila, and many of the genes have no close homologues in fungi, plants, or animals. Membrane transporters are particularly challenging to characterize because these proteins may be part of large families, and homology is not particularly useful in predicting substrate specificity. Here we show by heterologous expression in yeast that the P. parasitica protein PPTG_16698, Transporter of Putrescine and Spermidine (TOPAS1) is a polyamine transporter. Heterologous expression of this gene in yeast protects cells from the toxic effects of high levels of putrescine in the media, suggesting that TOPAS1 also acts to sequester polyamines within the cell. TOPAS1 has the fifth highest level of expression of all membrane transporters in the zoosporic stage of this pathogen. Since zoospores take up polyamines form the media this high level of expression hints at the importance of polyamine acquisition and compartmentation for this developmental stage. TOPAS1 is one of 13 highly conserved membrane transporters in P. parasitica, and orthologs to these proteins are found in other Phytophthora species. Members of this family are also found in other oomycetes, although there are no homologues to these proteins in other eukaryotes. 2017-08-02 English text Bowling Green State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=bgsu149874256456224 http://rave.ohiolink.edu/etdc/view?acc_num=bgsu149874256456224 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Molecular Biology polyamines conserved family phytophthora parasitica transporters |
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Molecular Biology polyamines conserved family phytophthora parasitica transporters Chakrabarti, Nilanjana Functional Analysis and Characterization of Transporter of Putrescine and Spermidine (TOPAS1) in Phytophthora parasitica |
| author |
Chakrabarti, Nilanjana |
| author_facet |
Chakrabarti, Nilanjana |
| author_sort |
Chakrabarti, Nilanjana |
| title |
Functional Analysis and Characterization of Transporter of Putrescine and Spermidine (TOPAS1) in Phytophthora parasitica |
| title_short |
Functional Analysis and Characterization of Transporter of Putrescine and Spermidine (TOPAS1) in Phytophthora parasitica |
| title_full |
Functional Analysis and Characterization of Transporter of Putrescine and Spermidine (TOPAS1) in Phytophthora parasitica |
| title_fullStr |
Functional Analysis and Characterization of Transporter of Putrescine and Spermidine (TOPAS1) in Phytophthora parasitica |
| title_full_unstemmed |
Functional Analysis and Characterization of Transporter of Putrescine and Spermidine (TOPAS1) in Phytophthora parasitica |
| title_sort |
functional analysis and characterization of transporter of putrescine and spermidine (topas1) in phytophthora parasitica |
| publisher |
Bowling Green State University / OhioLINK |
| publishDate |
2017 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu149874256456224 |
| work_keys_str_mv |
AT chakrabartinilanjana functionalanalysisandcharacterizationoftransporterofputrescineandspermidinetopas1inphytophthoraparasitica |
| _version_ |
1719452319067668480 |