Identification, Enumeration and Diversity of Nitrifying Bacteria in the Laurentian Great Lakes
| Main Author: | |
|---|---|
| Language: | English |
| Published: |
Bowling Green State University / OhioLINK
2012
|
| Subjects: | |
| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1351276518 |
| id |
ndltd-OhioLink-oai-etd.ohiolink.edu-bgsu1351276518 |
|---|---|
| record_format |
oai_dc |
| spelling |
ndltd-OhioLink-oai-etd.ohiolink.edu-bgsu13512765182021-08-03T05:30:09Z Identification, Enumeration and Diversity of Nitrifying Bacteria in the Laurentian Great Lakes Ray, Anirban Biology Ecology Environmental Science Limnology Microbiology Molecular Biology nitrification nitrate ammonia oxidation nitrite oxidation Lake Superior Lake Erie ammonia monooxygenase hydroxylamine oxidoreductase FISH <p>In the past 100 years the nitrate levels in Lake Superior have increased more than five times (Sterner et al. 2007). Based on stable isotope assays, previous research has shown that most of this nitrate is coming from in-lake nitrification process in the lake (Finlay et al. 2007), reflecting an imbalanced nitrogen cycle. By contrast, in Lake Erie the nitrate levels are declining. Lake Erie is the shallowest of the Great Lakes. The shallowness of the lake, the warmer temperature of the water, and nutrient inputs from urban and agricultural sources make it most biologically productive of the Great lakes. </p><p> Nitrification is a major process in the nitrogen cycle mainly carried out by the nitrifying microbial community (both Archaea and Bacteria), during which ammonia (NH3) is converted to Nitrite (NO2-) and then to nitrate (NO3-) by ammonia oxidizers (both Bacteria and Archaea) and Nitrite oxidizers (Bacteria only) respectively. Ammonia is oxidized by the enzyme ammonia monooxygenase, and hydroxylamine oxidoreductase (HAO). Nitrite (NO2-) converted to nitrate (NO3–) by Nitrite oxidizers (Bacteria) and enzyme nitrite oxidoreductase, carries this reaction. </p><p> In this thesis, I investigated the microbial nitrifier community structure by identifying and enumerating the ammonia-oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) present in these two lakes using the technique of fluorescence in-situ hybridization (FISH). This is the first study on Lake Superior and Lake Erie proving the overview of the abundance and diversity of these organisms. This study is focusing on understanding the nitrifying microbial community structure, contribution to other studies dealing with how these organisms function in the nitrogen cycling in these lakes. Therefore, the goal of this study is to provide measure of abundance of AOB and NOB in Lake Superior and Lake Erie as well as the diversity of AOB in these lakes.</p> 2012-11-09 English text Bowling Green State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1351276518 http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1351276518 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
| collection |
NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Biology Ecology Environmental Science Limnology Microbiology Molecular Biology nitrification nitrate ammonia oxidation nitrite oxidation Lake Superior Lake Erie ammonia monooxygenase hydroxylamine oxidoreductase FISH |
| spellingShingle |
Biology Ecology Environmental Science Limnology Microbiology Molecular Biology nitrification nitrate ammonia oxidation nitrite oxidation Lake Superior Lake Erie ammonia monooxygenase hydroxylamine oxidoreductase FISH Ray, Anirban Identification, Enumeration and Diversity of Nitrifying Bacteria in the Laurentian Great Lakes |
| author |
Ray, Anirban |
| author_facet |
Ray, Anirban |
| author_sort |
Ray, Anirban |
| title |
Identification, Enumeration and Diversity of Nitrifying Bacteria in the Laurentian Great Lakes |
| title_short |
Identification, Enumeration and Diversity of Nitrifying Bacteria in the Laurentian Great Lakes |
| title_full |
Identification, Enumeration and Diversity of Nitrifying Bacteria in the Laurentian Great Lakes |
| title_fullStr |
Identification, Enumeration and Diversity of Nitrifying Bacteria in the Laurentian Great Lakes |
| title_full_unstemmed |
Identification, Enumeration and Diversity of Nitrifying Bacteria in the Laurentian Great Lakes |
| title_sort |
identification, enumeration and diversity of nitrifying bacteria in the laurentian great lakes |
| publisher |
Bowling Green State University / OhioLINK |
| publishDate |
2012 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1351276518 |
| work_keys_str_mv |
AT rayanirban identificationenumerationanddiversityofnitrifyingbacteriainthelaurentiangreatlakes |
| _version_ |
1719420994505932800 |