Phospholipid Dependency of Membrane-Associated Pyridine Nucleotide-Utilizing and Succinate Dehydrogenase Activities of Adult Hymenolepis Diminuta (Cestoda) and Ascaris Suum (Nematoda)

Bibliographic Details
Main Author:	Breidenbach, Carl R.
Language:	English
Published:	Bowling Green State University / OhioLINK 2012
Subjects:	Biochemistry Biology Parasitology Hymenolepis diminuta Ascaris suum mitochondria parasite phospholipid phospholipase phospholipid depletion anaerobic electron transport tapeworm roundworm
Online Access:	http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1343921911

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spelling	ndltd-OhioLink-oai-etd.ohiolink.edu-bgsu13439219112021-08-03T05:30:09Z Phospholipid Dependency of Membrane-Associated Pyridine Nucleotide-Utilizing and Succinate Dehydrogenase Activities of Adult Hymenolepis Diminuta (Cestoda) and Ascaris Suum (Nematoda) Breidenbach, Carl R. Biochemistry Biology Parasitology Hymenolepis diminuta Ascaris suum mitochondria parasite phospholipid phospholipase phospholipid depletion anaerobic electron transport tapeworm roundworm <p>The adult intestinal cestode, Hymenolepis diminuta, is essentially anaerobic in its metabolism and generates ATP without the need for oxygen. H. diminuta relies upon a mitochondrial NADPH->NAD transhydrogenase to link the NADPH produced by the pyruvate-forming arm of the malate dismutation reaction, catalyzed by the mitochondrial “malic enzyme”, with the NADH-requiring, anaerobic electron transport system. The electron transport-coupled fumarate reductase serves to reduce fumarate, the terminal electron acceptor, to succinate. A phospholipid dependency was established previously with respect to the transhydrogenase, fumarate reductase, and the lesser NADH oxidase. Of the phospholipids assessed, the transhydrogenase exhibited a phosphatidylcholine preference.</p><p>The present study expands on prior findings by using phospholipase A1, A2, C and D, organic solvent, and ammonium sulfate treatments of H. diminuta mitochondrial membranes. Other reduced pyridine nucleotide-utilizing systems viz., NAD(P)H cytochrome c reductase, NADH->NAD transhydrogenation, NAD(P)H-, and lipoamide dehydrogenase activities as well as succinate dehydrogenase were evaluated. A comparative study also was undertaken by treatment with the phospholipases of isolated mitochondrial membranes from the anaerobic intestinal nematode, Ascaris suum.</p><p>The data presented indicate a phospholipid dependence not only of the previously reported systems, but of membrane-associated mitochondrial systems in H. diminuta and A. suum. H. diminuta NADH cytochrome c reductase displayed phospholipid dependence based on phospholipase A2 and C treatments, and a neutral lipid dependence based on organic solvent treatments. Ammonium sulfate fractionation had little effect. Succinate dehydrogenase activity displayed phospholipid dependence based on phospholipase C and organic solvent treatments. Ammonium sulfate fractionation decreased succinate dehydrogenase activity, but phosphatidylcholine supplementation further diminished activity.</p><p>A. suum NADH cytochrome c reductase, NADH oxidase and fumarate reductase systems exhibited phospholipid dependence based on phospholipase A2 and C treatments. Interestingly, A. suum succinate dehydrogenase appeared more resistant to phospholipase treatment than the corresponding H. diminuta system.</p> 2012-10-10 English text Bowling Green State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1343921911 http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1343921911 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection	NDLTD
language	English
sources	NDLTD
topic	Biochemistry Biology Parasitology Hymenolepis diminuta Ascaris suum mitochondria parasite phospholipid phospholipase phospholipid depletion anaerobic electron transport tapeworm roundworm
spellingShingle	Biochemistry Biology Parasitology Hymenolepis diminuta Ascaris suum mitochondria parasite phospholipid phospholipase phospholipid depletion anaerobic electron transport tapeworm roundworm Breidenbach, Carl R. Phospholipid Dependency of Membrane-Associated Pyridine Nucleotide-Utilizing and Succinate Dehydrogenase Activities of Adult Hymenolepis Diminuta (Cestoda) and Ascaris Suum (Nematoda)
author	Breidenbach, Carl R.
author_facet	Breidenbach, Carl R.
author_sort	Breidenbach, Carl R.
title	Phospholipid Dependency of Membrane-Associated Pyridine Nucleotide-Utilizing and Succinate Dehydrogenase Activities of Adult Hymenolepis Diminuta (Cestoda) and Ascaris Suum (Nematoda)
title_short	Phospholipid Dependency of Membrane-Associated Pyridine Nucleotide-Utilizing and Succinate Dehydrogenase Activities of Adult Hymenolepis Diminuta (Cestoda) and Ascaris Suum (Nematoda)
title_full	Phospholipid Dependency of Membrane-Associated Pyridine Nucleotide-Utilizing and Succinate Dehydrogenase Activities of Adult Hymenolepis Diminuta (Cestoda) and Ascaris Suum (Nematoda)
title_fullStr	Phospholipid Dependency of Membrane-Associated Pyridine Nucleotide-Utilizing and Succinate Dehydrogenase Activities of Adult Hymenolepis Diminuta (Cestoda) and Ascaris Suum (Nematoda)
title_full_unstemmed	Phospholipid Dependency of Membrane-Associated Pyridine Nucleotide-Utilizing and Succinate Dehydrogenase Activities of Adult Hymenolepis Diminuta (Cestoda) and Ascaris Suum (Nematoda)
title_sort	phospholipid dependency of membrane-associated pyridine nucleotide-utilizing and succinate dehydrogenase activities of adult hymenolepis diminuta (cestoda) and ascaris suum (nematoda)
publisher	Bowling Green State University / OhioLINK
publishDate	2012
url	http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1343921911
work_keys_str_mv	AT breidenbachcarlr phospholipiddependencyofmembraneassociatedpyridinenucleotideutilizingandsuccinatedehydrogenaseactivitiesofadulthymenolepisdiminutacestodaandascarissuumnematoda
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Phospholipid Dependency of Membrane-Associated Pyridine Nucleotide-Utilizing and Succinate Dehydrogenase Activities of Adult Hymenolepis Diminuta (Cestoda) and Ascaris Suum (Nematoda)

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