USING MOLECULAR PROBES TO DETECT CYANOBACTERIAL COMMUNITIES AND PHOSPHORUS UTILIZATION IN THE GREAT LAKES

Bibliographic Details
Main Author:	Kutovaya, Olga A.
Language:	English
Published:	Bowling Green State University / OhioLINK 2011
Subjects:	Ecology Environmental Science Freshwater Ecology Limnology Microbiology Molecular Biology picocyanobacteria phosphorus stress response genes adaptation
Online Access:	http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1304542118

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spelling	ndltd-OhioLink-oai-etd.ohiolink.edu-bgsu13045421182021-08-03T05:29:51Z USING MOLECULAR PROBES TO DETECT CYANOBACTERIAL COMMUNITIES AND PHOSPHORUS UTILIZATION IN THE GREAT LAKES Kutovaya, Olga A. Ecology Environmental Science Freshwater Ecology Limnology Microbiology Molecular Biology picocyanobacteria phosphorus stress response genes adaptation We examined the genetic potential of picocyanobacteria to recruit different sources of organic phosphorus, as well as their capacity to switch from phospholipids to sulfolipids in both Lake Erie and Lake Superior. The pelagic regions of Lake Superior and eastern Lake Erie are typically P-limited environments, and picocyanobacteria of the genus Synechococcus are the dominant primary producers during the summer. Specifically, the ability of endemic microbes to assimilate organic phosphates and phosphonates have been examined. As a proxy for their utilization of these substrates, the expression of two genes, phnD and phoX have been monitored. The phnD gene encodes the phosphonate binding protein of the ABC-type phosphonate transporter, whereas the phoX gene encodes a calcium-dependent alkaline phosphatase. To assess the ability of freshwater Synechococcus spp. to substitute sulfolipids for phospholipids, sqdX gene expression was monitored. The sqdX gene is a gene essential for sulfolipid biosynthesis and encodes the cyanobacterial sulfolipid synthase. We have developed PCR primers to detect the presence of all three genes in the endemic picocyanobacteria, and RT-PCR is being used to examine the patterns of expression that serve to assess the degree of P-stress experienced in the phytoplankton. To date, we show that the phnD gene is constitutively expressed, suggesting that freshwater picocyanobacteria are metabolizing exogenous phosphonate compounds in the severely P-limited environments. In contrast, phoX is regulated by P bioavailability in Great Lakes picocyanobacteria. We also provide evidence that sqdX is expressed during increased growth rates in phosphorus-replete conditions. 2011-06-21 English text Bowling Green State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1304542118 http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1304542118 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection	NDLTD
language	English
sources	NDLTD
topic	Ecology Environmental Science Freshwater Ecology Limnology Microbiology Molecular Biology picocyanobacteria phosphorus stress response genes adaptation
spellingShingle	Ecology Environmental Science Freshwater Ecology Limnology Microbiology Molecular Biology picocyanobacteria phosphorus stress response genes adaptation Kutovaya, Olga A. USING MOLECULAR PROBES TO DETECT CYANOBACTERIAL COMMUNITIES AND PHOSPHORUS UTILIZATION IN THE GREAT LAKES
author	Kutovaya, Olga A.
author_facet	Kutovaya, Olga A.
author_sort	Kutovaya, Olga A.
title	USING MOLECULAR PROBES TO DETECT CYANOBACTERIAL COMMUNITIES AND PHOSPHORUS UTILIZATION IN THE GREAT LAKES
title_short	USING MOLECULAR PROBES TO DETECT CYANOBACTERIAL COMMUNITIES AND PHOSPHORUS UTILIZATION IN THE GREAT LAKES
title_full	USING MOLECULAR PROBES TO DETECT CYANOBACTERIAL COMMUNITIES AND PHOSPHORUS UTILIZATION IN THE GREAT LAKES
title_fullStr	USING MOLECULAR PROBES TO DETECT CYANOBACTERIAL COMMUNITIES AND PHOSPHORUS UTILIZATION IN THE GREAT LAKES
title_full_unstemmed	USING MOLECULAR PROBES TO DETECT CYANOBACTERIAL COMMUNITIES AND PHOSPHORUS UTILIZATION IN THE GREAT LAKES
title_sort	using molecular probes to detect cyanobacterial communities and phosphorus utilization in the great lakes
publisher	Bowling Green State University / OhioLINK
publishDate	2011
url	http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1304542118
work_keys_str_mv	AT kutovayaolgaa usingmolecularprobestodetectcyanobacterialcommunitiesandphosphorusutilizationinthegreatlakes
_version_	1719420865762820096

USING MOLECULAR PROBES TO DETECT CYANOBACTERIAL COMMUNITIES AND PHOSPHORUS UTILIZATION IN THE GREAT LAKES

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