A Single Molecule Study of Calcium Effect on Nuclear Transport

A Single Molecule Study of Calcium Effect on Nuclear Transport

Bibliographic Details
Main Author: Sarma, Ashapurna
Language:English
Published: Bowling Green State University / OhioLINK 2010
Subjects:
Biology
Cellular Biology
Nuclear transport
calcium mediated nuclear transport
single-molecule imaging
Nuclear pore complex
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1282326584
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-bgsu12823265842021-08-03T05:29:32Z A Single Molecule Study of Calcium Effect on Nuclear Transport Sarma, Ashapurna Biology Cellular Biology Nuclear transport calcium mediated nuclear transport single-molecule imaging Nuclear pore complex Nuclear pore complexes (NPCs) embedded in the nuclear envelope (NE) is the sole pathway for direct communication between the cytoplasm and the nucleoplasm of eukaryotic cells. NPC allows unregulated passive diffusion of small molecules (< 20 kDa – 40kDa) and facilitated translocation of larger molecules (up to 500 MDa). More and more evidence suggests that calcium stored in the lumen of nuclear envelope and endoplasmic reticulum may further regulate nucleocytoplasmic transport. However with challenges in direct measurements of transport kinetics in the NPC, the calcium-regulated mechanism is still poorly understood. Here single-molecule fluorescence microscopy was used to characterize the nuclear pore permeability of passive diffusion and facilitated translocation under various calcium store concentrations. By snapshots of real-time transient movements of small molecules (10 kDa dextran) and large molecules (97 kDa protein -importin β1 (Imp β), through the NPCs, novel features under real-time trafficking conditions were observed that escaped detection by ensemble measurements. It was found that: i) transport rates cannot be used to reflect the change of nuclear pore permeability, which was mistakenly used in previous ensemble experiments; ii) transport rates for both passive diffusion and facilitated translocation can be significantly affected by the store calcium concentrations; and iii) nuclear pore permeability for passive diffusion is affected more by the amount of stored calcium than that for facilitated translocation. 2010-11-12 English text Bowling Green State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1282326584 http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1282326584 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Biology
Cellular Biology
Nuclear transport
calcium mediated nuclear transport
single-molecule imaging
Nuclear pore complex
spellingShingle Biology
Cellular Biology
Nuclear transport
calcium mediated nuclear transport
single-molecule imaging
Nuclear pore complex
Sarma, Ashapurna
A Single Molecule Study of Calcium Effect on Nuclear Transport
author Sarma, Ashapurna
author_facet Sarma, Ashapurna
author_sort Sarma, Ashapurna
title A Single Molecule Study of Calcium Effect on Nuclear Transport
title_short A Single Molecule Study of Calcium Effect on Nuclear Transport
title_full A Single Molecule Study of Calcium Effect on Nuclear Transport
title_fullStr A Single Molecule Study of Calcium Effect on Nuclear Transport
title_full_unstemmed A Single Molecule Study of Calcium Effect on Nuclear Transport
title_sort single molecule study of calcium effect on nuclear transport
publisher Bowling Green State University / OhioLINK
publishDate 2010
url http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1282326584
work_keys_str_mv AT sarmaashapurna asinglemoleculestudyofcalciumeffectonnucleartransport
AT sarmaashapurna singlemoleculestudyofcalciumeffectonnucleartransport
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