Production of Recombinant Carp Leptin and its Effects on Lipid Metabolism in the Common Carp (Cyprinus Carpio)
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University of Akron / OhioLINK
2012
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ndltd-OhioLink-oai-etd.ohiolink.edu-akron13421359532021-08-03T05:26:49Z Production of Recombinant Carp Leptin and its Effects on Lipid Metabolism in the Common Carp (Cyprinus Carpio) Copeland, Donald Lee Physiology The protein hormone leptin has emerged as a major contributor in the regulation of energy balance. However, most of our knowledge of leptin stems from mammalian studies that have focused primarily leptin's role in human pathologies (e.g. obesity). Little is known about how this hormone evolved and how it functions in more basal vertebrates. Recent efforts have identified the gene for leptin in many non-mammalian taxa including several fish species. Common carp (Cyprinus carpio) represents one such species and is an excellent system to study leptin because their otherwise well-studied physiology provides a plethora of context to interpret the effects of leptin. In particular, leptin has potent effects on lipolysis (i.e. triglyceride hydrolysis) and lipid oxidation (¿¿¿¿-oxidation of fatty acids) in mammals; a function that may be conserved in fishes. In order to evaluate the effects of leptin on lipid metabolism in carp, I first produced recombinant carp leptin-I using a bacterial expression system. Carp leptin was injected into the intraperitoneal cavity of koi (domesticated common carp) then, after a three hour recovery period, sampled for plasma and liver tissue. To evaluate the effects of leptin on lipolysis, plasma non-esterified fatty acids (NEFA) concentrations were measured. Additionally, hepatic enzyme activity of carnitine palmitoyltransferase-I (CPT) was measured to assess changes in fatty acid oxidation. Leptin injections increased plasma NEFA concentrations significantly but had no effect on hepatic CPT activity. These results suggest that leptin stimulates lipolysis but may not affect fatty acid oxidation as it does in mammals. I suggest that additional studies are needed to look at more specific indicators of lipid catabolism and oxidation, and higher more chronic doses of leptin should be used to thoroughly evaluate leptin's role in the lipid metabolism of fishes. 2012-08-02 English text University of Akron / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=akron1342135953 http://rave.ohiolink.edu/etdc/view?acc_num=akron1342135953 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Physiology |
| spellingShingle |
Physiology Copeland, Donald Lee Production of Recombinant Carp Leptin and its Effects on Lipid Metabolism in the Common Carp (Cyprinus Carpio) |
| author |
Copeland, Donald Lee |
| author_facet |
Copeland, Donald Lee |
| author_sort |
Copeland, Donald Lee |
| title |
Production of Recombinant Carp Leptin and its Effects on Lipid Metabolism in the Common Carp (Cyprinus Carpio) |
| title_short |
Production of Recombinant Carp Leptin and its Effects on Lipid Metabolism in the Common Carp (Cyprinus Carpio) |
| title_full |
Production of Recombinant Carp Leptin and its Effects on Lipid Metabolism in the Common Carp (Cyprinus Carpio) |
| title_fullStr |
Production of Recombinant Carp Leptin and its Effects on Lipid Metabolism in the Common Carp (Cyprinus Carpio) |
| title_full_unstemmed |
Production of Recombinant Carp Leptin and its Effects on Lipid Metabolism in the Common Carp (Cyprinus Carpio) |
| title_sort |
production of recombinant carp leptin and its effects on lipid metabolism in the common carp (cyprinus carpio) |
| publisher |
University of Akron / OhioLINK |
| publishDate |
2012 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=akron1342135953 |
| work_keys_str_mv |
AT copelanddonaldlee productionofrecombinantcarpleptinanditseffectsonlipidmetabolisminthecommoncarpcyprinuscarpio |
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