In Vitro Characterization Of Simvastatin Loaded Microspheres In The PolyRing Device

In Vitro Characterization Of Simvastatin Loaded Microspheres In The PolyRing Device

Bibliographic Details
Main Author: Vishwanathan, Anusha
Language:English
Published: University of Akron / OhioLINK 2008
Subjects:
Biomedical Research
Controlled Drug Delivery Sytems
Perivascular drug delivery
Simvastatin
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=akron1205934178
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-akron12059341782021-08-03T05:25:36Z In Vitro Characterization Of Simvastatin Loaded Microspheres In The PolyRing Device Vishwanathan, Anusha Biomedical Research Controlled Drug Delivery Sytems Perivascular drug delivery Simvastatin Intimal hyperplasia is the most common mechanism of failure for interventional and revascularization procedures like coronary artery bypass grafts (CABG), peripheral artery bypass surgery and dialysis access grafts. It is characterized by the thickening of intima within the artery wall. The reason for the thickening is due to the proliferation of medial vascular smooth muscle cells in the intima of the vessel wall. In a previous study, a novel targeted drug delivery system named the PolyRing was devised, using the drug cyclosporin A (CyA). The objective of our present research was to modify the previously established PolyRing system using the drug simvastatin, a hydroxymethylglutaryl coenzyme A (HMG-CoA) reductase inhibitor. The research hypotheses were that the loading of simvastatin in the PLGA microspheres could be optimized, and that there was controlled local release of the drug from the PolyRing device over a period of time. In this system, simvastatin was encapsulated within PLGA microspheres, which in turn were entrenched in a PEG block as a polymeric vascular wrap. The microspheres were prepared by an oil-in-water (o/w) emulsion technique. In order to achieve the maximum load, a total of eight runs with various formulation parameters including drug to polymer ratio, surfactant concentration, emulsification time and aqueous volume were conducted. Characterization of the microspheres was then completed to examine the surface morphology, particle size distribution, drug loading, encapsulation efficiency and the device in vitro release behavior. For this study, the null hypothesis was that there were no significant effects of the formulation parameters on encapsulation efficiency. Approximately 100µm size diameter spherical, smooth microspheres were obtained with no appreciable drug loss on the surface. The highest drug loading was observed to be 81.5 ± 6.3 µg drug/mg of microspheres. Only the drug to polymer ratio had significant effect on the encapsulation efficiency with the highest simvastatin encapsulated within the microspheres being 81 ± 9%. In vitro release behavior could not be determined due to the extremely low levels of drug detection in the release medium. Results from a series of experiments performed suggested that there was detection of simvastatin acid, an active metabolite of simvastatin. Alternate means for a suitable detection method have been proposed as future work. 2008-05-12 English text University of Akron / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=akron1205934178 http://rave.ohiolink.edu/etdc/view?acc_num=akron1205934178 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Biomedical Research
Controlled Drug Delivery Sytems
Perivascular drug delivery
Simvastatin
spellingShingle Biomedical Research
Controlled Drug Delivery Sytems
Perivascular drug delivery
Simvastatin
Vishwanathan, Anusha
In Vitro Characterization Of Simvastatin Loaded Microspheres In The PolyRing Device
author Vishwanathan, Anusha
author_facet Vishwanathan, Anusha
author_sort Vishwanathan, Anusha
title In Vitro Characterization Of Simvastatin Loaded Microspheres In The PolyRing Device
title_short In Vitro Characterization Of Simvastatin Loaded Microspheres In The PolyRing Device
title_full In Vitro Characterization Of Simvastatin Loaded Microspheres In The PolyRing Device
title_fullStr In Vitro Characterization Of Simvastatin Loaded Microspheres In The PolyRing Device
title_full_unstemmed In Vitro Characterization Of Simvastatin Loaded Microspheres In The PolyRing Device
title_sort in vitro characterization of simvastatin loaded microspheres in the polyring device
publisher University of Akron / OhioLINK
publishDate 2008
url http://rave.ohiolink.edu/etdc/view?acc_num=akron1205934178
work_keys_str_mv AT vishwanathananusha invitrocharacterizationofsimvastatinloadedmicrospheresinthepolyringdevice
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