Novel Diazeniumdiolates Nitric Oxide Donors and Devices for Biomedical Applications
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University of Akron / OhioLINK
2005
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ndltd-OhioLink-oai-etd.ohiolink.edu-akron11347462532021-08-03T05:24:40Z Novel Diazeniumdiolates Nitric Oxide Donors and Devices for Biomedical Applications Lopez, Marcos nitric oxide electrospinning leishmaniasis diabetes diabetes foot ulcers peripheral neuropathy diazeniumdiolates ethambutol electrospray ionization mass spectrometry <p>Nitric oxide (NO) mediates multiple physiological and pathophysiological processes in humans. In diabetic foot ulcers (DFU) and cutaneous Leishmaniasis (CL), which are two of the largest public health problems of greater world-wide importance due to its impact and the enormous social and economical repercussion, the use of NO has been recognized to also exert beneficial and effective results related to an adequate healing due to its antibiotic, vasodilator and promitogenic effect. For this purpose, we have developed a multilayer transdermal electrospun wound dressing which upon hydration allows the controlled release of nitric oxide from a NO<sub>2</sub><sup>-</sup>/ascorbic acid system encapsulated in polymer nanofibers. Using this approach, we have been successful in producing a wound dressing capable of delivering approximately 3 µmoles of NO/cm<sup>2</sup> and that maintains its activity after its sterilization with ethylene oxide. Double blinded clinical studies were performed to determine the efficacy of the nitric oxide releasing patch (NOP) in treating diabetic foot ulcers and leishmaniasis cutaneous. For the DFU, with daily application of the NOP, preliminary results have shown a complete re-epithelization, development of fibrous tissue and recovery of sensibility in the affected area in approximately 3-5 months. In the case of CL, the first cases analyzed have presented in an average of 15 days, a complete re-epithelization, negative parasitological results, and no re-infection after 6 months from the end of the treatment with daily application of the NOP. These preliminary results suggest that the use of the NOP may provide a low cost alternative and non-invasive therapy to treat these pathologies of world-wide importance.</p><p>In other approaches, such as surgical procedures, there is a need to develop novel biocompatible therapies to deliver NO to aid in the wound healing process. The diazeniumdiolates, which are a novel class of NO donors, are an excellent alternative because they have been shown to be more effective than any of the current NO donors due to their release of NO by hydrolysis and biocompatibility. Diazeniumdiolates of diaminobutane and ethylenediamine diesters were synthesized and characterized. Diazeniumdiolates out of ethambutol and ephedrine were also prepared. Properties of these diazeniumdiolates varied depending on their ester groups. At physiological conditions, the amount of NO released was assessed. The antibacterial effects of the ethambutol (ETBNO) and diethyl spermeate diazeniumdiolates were tested against strains of <i>S. aureous</i>, showing inhibition areas at low doses coated on small intestinal submucosa samples (SIS<sup>®</sup>). These diazeniumdiolates have potential biomedical applications such as biocompatible enhancers for various in vivo biomedical devices and antimicrobials.</p> 2005 English text University of Akron / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=akron1134746253 http://rave.ohiolink.edu/etdc/view?acc_num=akron1134746253 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
nitric oxide electrospinning leishmaniasis diabetes diabetes foot ulcers peripheral neuropathy diazeniumdiolates ethambutol electrospray ionization mass spectrometry |
| spellingShingle |
nitric oxide electrospinning leishmaniasis diabetes diabetes foot ulcers peripheral neuropathy diazeniumdiolates ethambutol electrospray ionization mass spectrometry Lopez, Marcos Novel Diazeniumdiolates Nitric Oxide Donors and Devices for Biomedical Applications |
| author |
Lopez, Marcos |
| author_facet |
Lopez, Marcos |
| author_sort |
Lopez, Marcos |
| title |
Novel Diazeniumdiolates Nitric Oxide Donors and Devices for Biomedical Applications |
| title_short |
Novel Diazeniumdiolates Nitric Oxide Donors and Devices for Biomedical Applications |
| title_full |
Novel Diazeniumdiolates Nitric Oxide Donors and Devices for Biomedical Applications |
| title_fullStr |
Novel Diazeniumdiolates Nitric Oxide Donors and Devices for Biomedical Applications |
| title_full_unstemmed |
Novel Diazeniumdiolates Nitric Oxide Donors and Devices for Biomedical Applications |
| title_sort |
novel diazeniumdiolates nitric oxide donors and devices for biomedical applications |
| publisher |
University of Akron / OhioLINK |
| publishDate |
2005 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=akron1134746253 |
| work_keys_str_mv |
AT lopezmarcos noveldiazeniumdiolatesnitricoxidedonorsanddevicesforbiomedicalapplications |
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1719419508341342208 |