PROTEOMIC ANALYSIS OF THE EFFECTS OF DIET IN ZEBRAFISH LIVER

PROTEOMIC ANALYSIS OF THE EFFECTS OF DIET IN ZEBRAFISH LIVER

Bibliographic Details
Main Author: Jury, David R.
Language:English
Published: University of Akron / OhioLINK 2005
Subjects:
Proteomics
Diet
Zebrafish
Liver
Calorie availability
Stress response
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=akron1129389691
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-akron11293896912021-08-03T05:24:40Z PROTEOMIC ANALYSIS OF THE EFFECTS OF DIET IN ZEBRAFISH LIVER Jury, David R. Proteomics Diet Zebrafish Liver Calorie availability Stress response Characterizing molecular signaling in lipid metabolism traditionally has not been investigated with an integrated global mechanism approach. The peroxisome proliferated-activated receptors (PPARs) are a family of transcription factors that in response to lipids orchestrate metabolism. It was hypothesized that a high-calorie diet in zebrafish would up-regulate activity of PPARs. Proteomics identified discrete focused bands with greater than a 200% difference between the high-calorie and low-calorie diet treatment groups. Protein bands were subsequently submitted for identification with mass spectrometry. A lower total number of discrete proteins were expressed in the high-calorie group, which can be interpreted as part of a general stress response. Specific proteins up-regulated indicative of stress were prohibitin, glutamate oxaloacetate transaminase, and glyoxylase II. Down-regulated proteins indicative of stress induced retarded growth included cytokeratin, brain type fatty acid binding protein, profilin 2, and histone. Other proteins exhibited expression patterns that did not fit into a predictable stress response. 2005 English text University of Akron / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=akron1129389691 http://rave.ohiolink.edu/etdc/view?acc_num=akron1129389691 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Proteomics
Diet
Zebrafish
Liver
Calorie availability
Stress response
spellingShingle Proteomics
Diet
Zebrafish
Liver
Calorie availability
Stress response
Jury, David R.
PROTEOMIC ANALYSIS OF THE EFFECTS OF DIET IN ZEBRAFISH LIVER
author Jury, David R.
author_facet Jury, David R.
author_sort Jury, David R.
title PROTEOMIC ANALYSIS OF THE EFFECTS OF DIET IN ZEBRAFISH LIVER
title_short PROTEOMIC ANALYSIS OF THE EFFECTS OF DIET IN ZEBRAFISH LIVER
title_full PROTEOMIC ANALYSIS OF THE EFFECTS OF DIET IN ZEBRAFISH LIVER
title_fullStr PROTEOMIC ANALYSIS OF THE EFFECTS OF DIET IN ZEBRAFISH LIVER
title_full_unstemmed PROTEOMIC ANALYSIS OF THE EFFECTS OF DIET IN ZEBRAFISH LIVER
title_sort proteomic analysis of the effects of diet in zebrafish liver
publisher University of Akron / OhioLINK
publishDate 2005
url http://rave.ohiolink.edu/etdc/view?acc_num=akron1129389691
work_keys_str_mv AT jurydavidr proteomicanalysisoftheeffectsofdietinzebrafishliver
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