The role of indigenously-associated abuscular mycorrhizal fungi as biofertilisers and biological disease-control agents in subsistence cultivation of morogo / Mohlapa Junior Sekoele

The study examined interactions between morogo plants, arbuscular mycorrhizal fungi (AMF) and Fusarium species. Morogo refers to traditional leafy vegetables that, together with maize porridge, are dominant staple foods in rural areas of the Limpopo Province such as the Dikgale Demographic Surveilla...

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Main Author: Sekoele, Mohlapa Junior
Published: North-West University 2009
Subjects:
Online Access:http://hdl.handle.net/10394/1263
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spelling ndltd-NWUBOLOKA1-oai-dspace.nwu.ac.za-10394-12632014-04-16T03:55:21ZThe role of indigenously-associated abuscular mycorrhizal fungi as biofertilisers and biological disease-control agents in subsistence cultivation of morogo / Mohlapa Junior SekoeleSekoele, Mohlapa JuniorArbuscular mycorrhizal fungi (AMF)FusariumMorogoCowpea (Vigna unguiculata)Biological growth enhancerBiocontrol agentSubsistence farmingTraditional / indigenous knowledgeThe study examined interactions between morogo plants, arbuscular mycorrhizal fungi (AMF) and Fusarium species. Morogo refers to traditional leafy vegetables that, together with maize porridge, are dominant staple foods in rural areas of the Limpopo Province such as the Dikgale Demographic Surveillance Site (DDSS). Morogo plants grow either as weeds (often among maize), occur naturally in the field or are cultivated as subsistence crops by rural communities. Botanical species of morogo plants consumed in the DDSS were determined. Colonisation of morogo plant roots by AMF and Fusarium species composition in the immediate soil environment were investigated in four of eight DDSS subsistence communities, Isolated AMF were shown to belong to the genera Acaulospora and Glomus. Twelve Fusarium species were isolated from soil among which Fusariurn verticilliodes and Fusarium proliferaturn occurred predominantly. Greenhouse pot trials were conducted to examine the effect of AMF on morogo plant growth (cowpea; Mgna unguiculata) and Fusarium proliferatum levels in soil, Interaction between plants and AMF, as well as tripartite interactions of cowpea plants, AMF and Fusarium proliferatum were investigated. Non-inoculated cowpea plants served as controls for the following inoculations of cowpea in pots: (i) Fusarium proliferatum; (ii) commercial AMF from Mycoroot (PTY) Ltd. (a mixture of selected indigenous Glomus spp referred to commercial AMF for the purpose of this study); (iii) indigenous AMF obtained from DDSS soil (referred to iocal AMF for the purpose of this study); (iv) commercial AMF plus Fusarium proliferatum; (v) local AMF plus Fusariurn proliferatum. Results showed reduced root colonization by local as well as commercial AMF when Fusarium proliferatum were present. Local AMF significantly enhanced cowpea growth while commercial AMF apparently reduced the level of Fusarium proliferatum in the rhizosphere and surrounding soil. Results suggest that AMF may have potential as biological growth enhancers and bioprotective agents against Fusarium proliferatum.Thesis (M. Environmental Science (Water Science))--North-West University, Potchefstroom Campus, 2007.North-West University2009-03-02T15:14:49Z2009-03-02T15:14:49Z2006Thesishttp://hdl.handle.net/10394/1263
collection NDLTD
sources NDLTD
topic Arbuscular mycorrhizal fungi (AMF)
Fusarium
Morogo
Cowpea (Vigna unguiculata)
Biological growth enhancer
Biocontrol agent
Subsistence farming
Traditional / indigenous knowledge
spellingShingle Arbuscular mycorrhizal fungi (AMF)
Fusarium
Morogo
Cowpea (Vigna unguiculata)
Biological growth enhancer
Biocontrol agent
Subsistence farming
Traditional / indigenous knowledge
Sekoele, Mohlapa Junior
The role of indigenously-associated abuscular mycorrhizal fungi as biofertilisers and biological disease-control agents in subsistence cultivation of morogo / Mohlapa Junior Sekoele
description The study examined interactions between morogo plants, arbuscular mycorrhizal fungi (AMF) and Fusarium species. Morogo refers to traditional leafy vegetables that, together with maize porridge, are dominant staple foods in rural areas of the Limpopo Province such as the Dikgale Demographic Surveillance Site (DDSS). Morogo plants grow either as weeds (often among maize), occur naturally in the field or are cultivated as subsistence crops by rural communities. Botanical species of morogo plants consumed in the DDSS were determined. Colonisation of morogo plant roots by AMF and Fusarium species composition in the immediate soil environment were investigated in four of eight DDSS subsistence communities, Isolated AMF were shown to belong to the genera Acaulospora and Glomus. Twelve Fusarium species were isolated from soil among which Fusariurn verticilliodes and Fusarium proliferaturn occurred predominantly. Greenhouse pot trials were conducted to examine the effect of AMF on morogo plant growth (cowpea; Mgna unguiculata) and Fusarium proliferatum levels in soil, Interaction between plants and AMF, as well as tripartite interactions of cowpea plants, AMF and Fusarium proliferatum were investigated. Non-inoculated cowpea plants served as controls for the following inoculations of cowpea in pots: (i) Fusarium proliferatum; (ii) commercial AMF from Mycoroot (PTY) Ltd. (a mixture of selected indigenous Glomus spp referred to commercial AMF for the purpose of this study); (iii) indigenous AMF obtained from DDSS soil (referred to iocal AMF for the purpose of this study); (iv) commercial AMF plus Fusarium proliferatum; (v) local AMF plus Fusariurn proliferatum. Results showed reduced root colonization by local as well as commercial AMF when Fusarium proliferatum were present. Local AMF significantly enhanced cowpea growth while commercial AMF apparently reduced the level of Fusarium proliferatum in the rhizosphere and surrounding soil. Results suggest that AMF may have potential as biological growth enhancers and bioprotective agents against Fusarium proliferatum. === Thesis (M. Environmental Science (Water Science))--North-West University, Potchefstroom Campus, 2007.
author Sekoele, Mohlapa Junior
author_facet Sekoele, Mohlapa Junior
author_sort Sekoele, Mohlapa Junior
title The role of indigenously-associated abuscular mycorrhizal fungi as biofertilisers and biological disease-control agents in subsistence cultivation of morogo / Mohlapa Junior Sekoele
title_short The role of indigenously-associated abuscular mycorrhizal fungi as biofertilisers and biological disease-control agents in subsistence cultivation of morogo / Mohlapa Junior Sekoele
title_full The role of indigenously-associated abuscular mycorrhizal fungi as biofertilisers and biological disease-control agents in subsistence cultivation of morogo / Mohlapa Junior Sekoele
title_fullStr The role of indigenously-associated abuscular mycorrhizal fungi as biofertilisers and biological disease-control agents in subsistence cultivation of morogo / Mohlapa Junior Sekoele
title_full_unstemmed The role of indigenously-associated abuscular mycorrhizal fungi as biofertilisers and biological disease-control agents in subsistence cultivation of morogo / Mohlapa Junior Sekoele
title_sort role of indigenously-associated abuscular mycorrhizal fungi as biofertilisers and biological disease-control agents in subsistence cultivation of morogo / mohlapa junior sekoele
publisher North-West University
publishDate 2009
url http://hdl.handle.net/10394/1263
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