Signal amplification by pretargeting with bispecifc antibody for highly sensitive in vitro immunofluorescence imaging
Monoclonal antibodies have been widely used in biological experiments and clinical therapeutics for sometime, however, use of directly labeled antibodies with fluorophores or therapeutics has been limited by nonspecific accumulation of antibody-drug-conjugates leading to low target to background rat...
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| Online Access: | http://hdl.handle.net/2047/D20198622 |
| Summary: | Monoclonal antibodies have been widely used in biological experiments and clinical therapeutics for sometime, however, use of directly labeled antibodies with fluorophores or therapeutics has been limited by nonspecific accumulation of antibody-drug-conjugates leading to low target to background ratios. Rhodamine B isothiocyanate (RITC) is widely used as a fluorescent tag in a variety of biological experiments. However, its major shortcoming is its self-quenching properties
when high specific activity reagents are needed. |
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