Genetic insight into the function of Pcp1p, a mitochondrial rhomboid protease
<p>Rhomboid peptidases are conserved intramembrane serine proteases with mitochondrial family members being involved in mitochondrial dynamics and apoptosis. The <i>Saccharomyces cerevisiae</i> mitochondrial rhomboid, Pcp1p, catalyzes the cleavage of two substrates: Ccp1p, which br...
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ndltd-MSSTATE-oai-library.msstate.edu-etd-02282013-1902112015-03-17T15:54:59Z Genetic insight into the function of Pcp1p, a mitochondrial rhomboid protease Xiao, Ningyu Biological Sciences <p>Rhomboid peptidases are conserved intramembrane serine proteases with mitochondrial family members being involved in mitochondrial dynamics and apoptosis. The <i>Saccharomyces cerevisiae</i> mitochondrial rhomboid, Pcp1p, catalyzes the cleavage of two substrates: Ccp1p, which breaks down reactive oxygen species, and Mgm1p, a GTPase mediating mitochondrial fusion events. As an initial approach to determine the structural basis of Pcp1p activity, a screen to identify temperature sensitive alleles of <i>PCP1</i> was performed using hydroxylamine mutagenesis. Eight mutants were identified from a pool of 30,000 colonies that exhibited either temperature sensitive growth or respiratory defects. These mutants also exhibited defects in Mgm1p and Ccp1p processing and some degree of abnormal mitochondrial morphology. The majority of amino acid changes occurred within the fourth and sixth transmembrane domains of Pcp1p, the location of the active site serine and histidine residues, supporting a role for these transmembrane helices in Pcp1p activity. </p> Donna M. Gordon MSSTATE 2013-04-23 text application/pdf http://sun.library.msstate.edu/ETD-db/theses/available/etd-02282013-190211/ http://sun.library.msstate.edu/ETD-db/theses/available/etd-02282013-190211/ en restricted I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, Dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Mississippi State University Libraries or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, Dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, Dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, Dissertation, or project report. |
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Biological Sciences Xiao, Ningyu Genetic insight into the function of Pcp1p, a mitochondrial rhomboid protease |
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<p>Rhomboid peptidases are conserved intramembrane serine proteases with mitochondrial family members being involved in mitochondrial dynamics and apoptosis. The <i>Saccharomyces cerevisiae</i> mitochondrial rhomboid, Pcp1p, catalyzes the cleavage of two substrates: Ccp1p, which breaks down reactive oxygen species, and Mgm1p, a GTPase mediating mitochondrial fusion events. As an initial approach to determine the structural basis of Pcp1p activity, a screen to identify temperature sensitive alleles of <i>PCP1</i> was performed using hydroxylamine mutagenesis. Eight mutants were identified from a pool of 30,000 colonies that exhibited either temperature sensitive growth or respiratory defects. These mutants also exhibited defects in Mgm1p and Ccp1p processing and some degree of abnormal mitochondrial morphology. The majority of amino acid changes occurred within the fourth and sixth transmembrane domains of Pcp1p, the location of the active site serine and histidine residues, supporting a role for these transmembrane helices in Pcp1p activity. </p> |
author2 |
Donna M. Gordon |
author_facet |
Donna M. Gordon Xiao, Ningyu |
author |
Xiao, Ningyu |
author_sort |
Xiao, Ningyu |
title |
Genetic insight into the function of Pcp1p, a mitochondrial rhomboid protease |
title_short |
Genetic insight into the function of Pcp1p, a mitochondrial rhomboid protease |
title_full |
Genetic insight into the function of Pcp1p, a mitochondrial rhomboid protease |
title_fullStr |
Genetic insight into the function of Pcp1p, a mitochondrial rhomboid protease |
title_full_unstemmed |
Genetic insight into the function of Pcp1p, a mitochondrial rhomboid protease |
title_sort |
genetic insight into the function of pcp1p, a mitochondrial rhomboid protease |
publisher |
MSSTATE |
publishDate |
2013 |
url |
http://sun.library.msstate.edu/ETD-db/theses/available/etd-02282013-190211/ |
work_keys_str_mv |
AT xiaoningyu geneticinsightintothefunctionofpcp1pamitochondrialrhomboidprotease |
_version_ |
1716731854404452352 |