Innovative approaches to assessing seed quality in Brassicas

• Main Author: Larkin, Jodi
• Other Authors: McVetty, Peter (Plant Science)
• Published: 2012
• Subjects: Brassica napus; chlorophyll concentration; erucic acid genes; near infrared reflectance spectroscopy; molecular markers
• Online Access: http://hdl.handle.net/1993/5230

Brassica napus is grown as an edible oil (canola) and an industrial oil (HEAR). Its fatty acid profile and chlorophyll concentration affect the quality. It is important to develop accurate and efficient methods to evaluate these traits. The first objective of this study was to improve the single nucleotide polymorphism (SNP) and sequence characterized amplified region (SCAR) molecular markers for erucic acid genotypes in the Bn-FAE1.1 and Bn-FAE1.2 genes in the A and C genomes in Brassica napus originally developed by Rahman et al. (2008). When put into practice, the error rate was unacceptably high. With the modifications that were made to the protocols, the overall accuracy remained relatively consistent indicating that further improvements are still required. The second objective was to develop a near infrared reflectance (NIR) based calibration equation for chlorophyll concentration in whole Brassica napus seeds. In this case, an equation was successfully created.