Biodiversity of the Muscidae (Diptera) from Churchill, Manitoba, Canada, with taxonomic issues revealed or resolved by DNA barcoding
A 2007 survey of muscid flies from Churchill, Manitoba, Canada, yielded 155 species. The diversity of this contemporary assemblage was compared to that of an historical (pre-1965) assemblage. Few differences were found between assemblages for material collected by net sweeping and most non-overlappi...
Main Author: | |
---|---|
Other Authors: | |
Published: |
2012
|
Subjects: | |
Online Access: | http://hdl.handle.net/1993/5029 |
id |
ndltd-MANITOBA-oai-mspace.lib.umanitoba.ca-1993-5029 |
---|---|
record_format |
oai_dc |
spelling |
ndltd-MANITOBA-oai-mspace.lib.umanitoba.ca-1993-50292014-01-31T03:32:43Z Biodiversity of the Muscidae (Diptera) from Churchill, Manitoba, Canada, with taxonomic issues revealed or resolved by DNA barcoding Renaud, Anais Krystel Savage, Jade (Bishop's University, Biological Sciences) Galloway, Terry (Entomology) Piercey-Normore, Michele (Biological Sciences) Smith, Marjorie (Agriculture and Agri-Food Canada) Insects Arctic A 2007 survey of muscid flies from Churchill, Manitoba, Canada, yielded 155 species. The diversity of this contemporary assemblage was compared to that of an historical (pre-1965) assemblage. Few differences were found between assemblages for material collected by net sweeping and most non-overlapping species between time periods were rare in samples and/or collected by different methods. DNA barcoding was used as a tool to assist with the identification of Muscidae specimens. Performance of the cytochrome c oxidase subunit 1 gene (COI) in the discrimination of muscid species was assessed and correspondence levels of 98.6% were established between species limits recovered by DNA barcode clusters and morphology. I conclude that the great majority of species limits currently accepted in the literature are adequate for Muscidae and that DNA barcoding is a useful identification tool for this family. 2012-01-05T16:34:01Z 2012-01-05T16:34:01Z 2012-01-05 http://hdl.handle.net/1993/5029 |
collection |
NDLTD |
sources |
NDLTD |
topic |
Insects Arctic |
spellingShingle |
Insects Arctic Renaud, Anais Krystel Biodiversity of the Muscidae (Diptera) from Churchill, Manitoba, Canada, with taxonomic issues revealed or resolved by DNA barcoding |
description |
A 2007 survey of muscid flies from Churchill, Manitoba, Canada, yielded 155 species. The diversity of this contemporary assemblage was compared to that of an historical (pre-1965) assemblage. Few differences were found between assemblages for material collected by net sweeping and most non-overlapping species between time periods were rare in samples and/or collected by different methods. DNA barcoding was used as a tool to assist with the identification of Muscidae specimens. Performance of the cytochrome c oxidase subunit 1 gene (COI) in the discrimination of muscid species was assessed and correspondence levels of 98.6% were established between species limits recovered by DNA barcode clusters and morphology. I conclude that the great majority of species limits currently accepted in the literature are adequate for Muscidae and that DNA barcoding is a useful identification tool for this family. |
author2 |
Savage, Jade (Bishop's University, Biological Sciences) Galloway, Terry (Entomology) |
author_facet |
Savage, Jade (Bishop's University, Biological Sciences) Galloway, Terry (Entomology) Renaud, Anais Krystel |
author |
Renaud, Anais Krystel |
author_sort |
Renaud, Anais Krystel |
title |
Biodiversity of the Muscidae (Diptera) from Churchill, Manitoba, Canada, with taxonomic issues revealed or resolved by DNA barcoding |
title_short |
Biodiversity of the Muscidae (Diptera) from Churchill, Manitoba, Canada, with taxonomic issues revealed or resolved by DNA barcoding |
title_full |
Biodiversity of the Muscidae (Diptera) from Churchill, Manitoba, Canada, with taxonomic issues revealed or resolved by DNA barcoding |
title_fullStr |
Biodiversity of the Muscidae (Diptera) from Churchill, Manitoba, Canada, with taxonomic issues revealed or resolved by DNA barcoding |
title_full_unstemmed |
Biodiversity of the Muscidae (Diptera) from Churchill, Manitoba, Canada, with taxonomic issues revealed or resolved by DNA barcoding |
title_sort |
biodiversity of the muscidae (diptera) from churchill, manitoba, canada, with taxonomic issues revealed or resolved by dna barcoding |
publishDate |
2012 |
url |
http://hdl.handle.net/1993/5029 |
work_keys_str_mv |
AT renaudanaiskrystel biodiversityofthemuscidaedipterafromchurchillmanitobacanadawithtaxonomicissuesrevealedorresolvedbydnabarcoding |
_version_ |
1716629090339913728 |