Biodiversity of the Muscidae (Diptera) from Churchill, Manitoba, Canada, with taxonomic issues revealed or resolved by DNA barcoding

A 2007 survey of muscid flies from Churchill, Manitoba, Canada, yielded 155 species. The diversity of this contemporary assemblage was compared to that of an historical (pre-1965) assemblage. Few differences were found between assemblages for material collected by net sweeping and most non-overlappi...

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Main Author: Renaud, Anais Krystel
Other Authors: Savage, Jade (Bishop's University, Biological Sciences) Galloway, Terry (Entomology)
Published: 2012
Subjects:
Online Access:http://hdl.handle.net/1993/5029
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spelling ndltd-MANITOBA-oai-mspace.lib.umanitoba.ca-1993-50292014-01-31T03:32:43Z Biodiversity of the Muscidae (Diptera) from Churchill, Manitoba, Canada, with taxonomic issues revealed or resolved by DNA barcoding Renaud, Anais Krystel Savage, Jade (Bishop's University, Biological Sciences) Galloway, Terry (Entomology) Piercey-Normore, Michele (Biological Sciences) Smith, Marjorie (Agriculture and Agri-Food Canada) Insects Arctic A 2007 survey of muscid flies from Churchill, Manitoba, Canada, yielded 155 species. The diversity of this contemporary assemblage was compared to that of an historical (pre-1965) assemblage. Few differences were found between assemblages for material collected by net sweeping and most non-overlapping species between time periods were rare in samples and/or collected by different methods. DNA barcoding was used as a tool to assist with the identification of Muscidae specimens. Performance of the cytochrome c oxidase subunit 1 gene (COI) in the discrimination of muscid species was assessed and correspondence levels of 98.6% were established between species limits recovered by DNA barcode clusters and morphology. I conclude that the great majority of species limits currently accepted in the literature are adequate for Muscidae and that DNA barcoding is a useful identification tool for this family. 2012-01-05T16:34:01Z 2012-01-05T16:34:01Z 2012-01-05 http://hdl.handle.net/1993/5029
collection NDLTD
sources NDLTD
topic Insects
Arctic
spellingShingle Insects
Arctic
Renaud, Anais Krystel
Biodiversity of the Muscidae (Diptera) from Churchill, Manitoba, Canada, with taxonomic issues revealed or resolved by DNA barcoding
description A 2007 survey of muscid flies from Churchill, Manitoba, Canada, yielded 155 species. The diversity of this contemporary assemblage was compared to that of an historical (pre-1965) assemblage. Few differences were found between assemblages for material collected by net sweeping and most non-overlapping species between time periods were rare in samples and/or collected by different methods. DNA barcoding was used as a tool to assist with the identification of Muscidae specimens. Performance of the cytochrome c oxidase subunit 1 gene (COI) in the discrimination of muscid species was assessed and correspondence levels of 98.6% were established between species limits recovered by DNA barcode clusters and morphology. I conclude that the great majority of species limits currently accepted in the literature are adequate for Muscidae and that DNA barcoding is a useful identification tool for this family.
author2 Savage, Jade (Bishop's University, Biological Sciences) Galloway, Terry (Entomology)
author_facet Savage, Jade (Bishop's University, Biological Sciences) Galloway, Terry (Entomology)
Renaud, Anais Krystel
author Renaud, Anais Krystel
author_sort Renaud, Anais Krystel
title Biodiversity of the Muscidae (Diptera) from Churchill, Manitoba, Canada, with taxonomic issues revealed or resolved by DNA barcoding
title_short Biodiversity of the Muscidae (Diptera) from Churchill, Manitoba, Canada, with taxonomic issues revealed or resolved by DNA barcoding
title_full Biodiversity of the Muscidae (Diptera) from Churchill, Manitoba, Canada, with taxonomic issues revealed or resolved by DNA barcoding
title_fullStr Biodiversity of the Muscidae (Diptera) from Churchill, Manitoba, Canada, with taxonomic issues revealed or resolved by DNA barcoding
title_full_unstemmed Biodiversity of the Muscidae (Diptera) from Churchill, Manitoba, Canada, with taxonomic issues revealed or resolved by DNA barcoding
title_sort biodiversity of the muscidae (diptera) from churchill, manitoba, canada, with taxonomic issues revealed or resolved by dna barcoding
publishDate 2012
url http://hdl.handle.net/1993/5029
work_keys_str_mv AT renaudanaiskrystel biodiversityofthemuscidaedipterafromchurchillmanitobacanadawithtaxonomicissuesrevealedorresolvedbydnabarcoding
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