Aerobic bioremoval of CAHs and BTEX from contaminated soil

Aerobic bioremoval of CAHs and BTEX from contaminated soil

University of Macau === Faculty of Science and Technology === Department of Civil and Environmental Engineering

Bibliographic Details
Main Author: Li, Jun Hui
Language:English
Published: University of Macau 2012
Subjects:
Bioremediation
Environmental engineering
Civil Engineering > Department of Civil and Environmental Engineering
Online Access:http://umaclib3.umac.mo/record=b2590543
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spelling ndltd-MACAU-oai-libdigital.umac.mo-b25905432013-01-07T23:07:52Z2012http://umaclib3.umac.mo/record=b2590543UM_THESESUniversity of MacauFaculty of Science and TechnologyDepartment of Civil and Environmental EngineeringUniversity of MacauBioremediationEnvironmental engineeringCivil Engineering -- Department of Civil and Environmental EngineeringengLi, Jun HuiAerobic bioremoval of CAHs and BTEX from contaminated soilChlorinated aliphatic hydrocarbons and monoaromatic hydrocarbons are major soil and water pollutants as a result of their widespread anthropogenic use. Soil contamination is of particular concern as it results in offsite contamination of groundwater and surface waters by migration of contaminants through and with soil materials. Co-contamination of soil with chlorinated aliphatic hydrocarbons and monoaromatic hydrocarbons allows for the application of aerobic bioremediation to achieve the simultaneous mineralization of both types of compounds. Bench scale laboratory experiments were run to select and enrich indigenous microbial isolates capable of cometabolizing two representative chlorinated aliphatic hydrocarbons, cis-1.2-dichloroethylene (cis-DCE) and trichloroethylene (TCE), mixed with benzene, toluene, ethylbenzene, and three isomers of xylene (BTEX) provided as substrates. The well-characterized toluene-oxidizing bacteria, Pseudomonas plecoglossicida, as well as 7 unidentified strains of bacteria isolated from potentially contaminated soil and sludge samples, were examined for their ability to degrade mixtures of BTEX, TCE or cis-DCE in liquid culture. This process was used as an initial screen to select the best bacterial isolate for further investigation. Based on results of these experiments, Pseudomonas plecoglossicida, showing higher mineralization extent of the mixure, especially TCE and cis-DCE, was chosen to investigate further. The aerobic simultaneous bioremoval of the mixture of TCE, cis-DCE, and BTEX was studied under different conditions through laboratory-scale batch experiments in 6-day incubation. Studies were performed on selected parameters (soil physicochemical properties—pH, temperature, and moisture; nutrient concentrations—nitrogen and phosphorus; contaminant properties—contaminant concentration, ratio of growth substrate (BTEX) to nongrowth substrate (chloroethenes); microbial inoculation amount) and optimization carried out to improve TCE/cis-DCE/BTEX mixture removal. The central composite design (CCD) together with the response surface methodology (RSM) was applied to evaluate the interactive effects of the parameters, as well as the optimal bioremoval conditions. The bioremoval of the mixture varied, depending on the conditions, and interactions among these compounds, especially growth and nongrowth substrate, were complicated. The presence of toluene, nonetheless, enhanced the bioremediation of other compounds unanimously. Overall, the methods and technologies presented here can be good candidates for bioremediation of the mixed wastes-contaminated sites.
collection NDLTD
language English
sources NDLTD
topic Bioremediation
Environmental engineering
Civil Engineering -- Department of Civil and Environmental Engineering
spellingShingle Bioremediation
Environmental engineering
Civil Engineering -- Department of Civil and Environmental Engineering
Li, Jun Hui
Aerobic bioremoval of CAHs and BTEX from contaminated soil
description University of Macau === Faculty of Science and Technology === Department of Civil and Environmental Engineering
author Li, Jun Hui
author_facet Li, Jun Hui
author_sort Li, Jun Hui
title Aerobic bioremoval of CAHs and BTEX from contaminated soil
title_short Aerobic bioremoval of CAHs and BTEX from contaminated soil
title_full Aerobic bioremoval of CAHs and BTEX from contaminated soil
title_fullStr Aerobic bioremoval of CAHs and BTEX from contaminated soil
title_full_unstemmed Aerobic bioremoval of CAHs and BTEX from contaminated soil
title_sort aerobic bioremoval of cahs and btex from contaminated soil
publisher University of Macau
publishDate 2012
url http://umaclib3.umac.mo/record=b2590543
work_keys_str_mv AT lijunhui aerobicbioremovalofcahsandbtexfromcontaminatedsoil
_version_ 1716479269662621696

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