Production of conjugated linoleic acid and conjugated linolenic acid by Bifidobacterium breve JKL03 and its application

• Main Author: Jung, Yun-Kyoung, 1979-
• Format: Others
• Language: en
• Published: McGill University 2005
• Subjects: Linoleic acid > Metabolism.; Linolenic acids > Metabolism.; Bifidobacterium > Industrial applications.
• Online Access: http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=82258

Conjugated linoleic acid (CLA) is predominantly found in foods of ruminant origin such as milk and processed cheese, and has gained much interest recently due to its beneficial health and biological effects on animals and humans. === The bioconversion of linoleic acid (LA) and linolenic acid (LNA) by a selected Bifidobacterium from healthy infant feces was studied. Bifidobacterium breve JKL03 had the ability to convert linolenic acid (0.2 mg/ml) to CLNA in fermentation of skim milk medium for 24 h up to a yield of 72.0% (up to 74.7% under aerobic conditions) and linoleic acid (0.2 mg/ml) into CLA by fermentation in skim milk medium for 24 h up to a yield of 23.9% (up to 28.0% under aerobic conditions). === B. breve JKL03 was also co-fermented with Lactobacillus acidophilus (NCFMRTM strain), a commonly added starter culture, to observe the resulting effects on growth during fermentation for yogurt production. Fermentation of LNA in skim milk with B. breve JKL03 and L. acidophilus (NCFM) maintained high CLNA production level. On the other hand, CLA production in the same media with both strains did not exhibit as high level as with the single B. breve. === These results are important for the advancement of knowledge on the production of CLA and CLNA in dairy products and for knowledge on the basic metabolic mechanisms for such conversion.