Localization and characterization of an ectodermal protein of sea urchin embryos
In the urchin Strongylocentrotus purpuratus, the expression of the Spec3 gene is associated with the growth of cilia, one of the first morphogenetic events during development. The product of this gene was characterized using an antiserum raised against a peptide corresponding to the predicted amino-...
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| Format: | Others |
| Language: | en |
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McGill University
1989
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| Online Access: | http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=59519 |
| Summary: | In the urchin Strongylocentrotus purpuratus, the expression of the Spec3 gene is associated with the growth of cilia, one of the first morphogenetic events during development. The product of this gene was characterized using an antiserum raised against a peptide corresponding to the predicted amino-terminal portion of the protein. This thesis describes the localization of the Spec3 protein at different stages during embryonic development. Immunocytochemistry indicated that the protein is associated with cilia and Golgi complexes of ectodermal cells. Agents that inhibit protein synthesis and Golgi secretion also altered its normal distribution. Fractionation of cilia and immunoblotting indicate that the protein is associated with the ciliary axoneme and that it behaves as a large aggregate. |
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